Thermo Fisher Scientific Phospho-ERK1/ERK2 (Thr202, Tyr204) Polyclonal Antibody
Phospho-ERK1/ERK2 (Thr202, Tyr204) 항체로 인간, 마우스, 랫트 시료에서 ERK1/2 MAP 키나아제의 인산화 형태 검출에 사용. WB, IHC, ICC에 적합하며, 고순도의 Rabbit Polyclonal 형식. 장기 보관 시 -20°C 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:4,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:2,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Phosphorylated peptide (pT202-E-pY204) based on human Erk1/2 MAP Kinase |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.3 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C (short term); for long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2234967 |
Product Specific Information
PA1-9591 detects Phospho-Erk1/2 MAP Kinase in human, mouse, and rat samples.
It has been successfully used in Western blot, immunohistochemistry, and immunocytochemistry applications.
By Western blot, PA1-9591 detects 42 kDa and 44 kDa proteins representing Phospho-Erk1/2 MAP Kinase.
The immunogen is a phosphorylated peptide (pT202-E-pY204) based on human Erk1/2 MAP Kinase.
Target Information
ERK1 and ERK2 are widely expressed kinases involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells.
These kinases are activated by various stimuli such as growth factors, cytokines, viral infections, and ligands for G protein-coupled receptors.
Upon activation, ERK1/2 phosphorylate cytoplasmic targets or translocate to the nucleus to activate transcription factors such as c-Fos and Elk-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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