Thermo Fisher Scientific Phospho-ERK1/ERK2 (Thr202, Tyr204) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:4,000

-

Immunohistochemistry (IHC)

1:500-1:2,000

-

Immunocytochemistry (ICC/IF)

1:500-1:2,000

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Phosphorylated peptide (pT202-E-pY204) based on human Erk1/2 MAP Kinase if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ERK1/2,uniProtId:P27361-1,ncbiNodeId:9606,antigenRange:202|204,antigenLength:379,antigenImageFileName:PA1-9591_ERK12_P27361-1_Rabbit.svg,antigenImageFileNamePDP:PA1-9591_ERK12_P27361-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:ERK1/2,uniProtId:P28482-1,ncbiNodeId:9606,antigenRange:202|204,antigenLength:360,antigenImageFileName:PA1-9591_ERK12_P28482-1_Rabbit.svg,antigenImageFileNamePDP:PA1-9591_ERK12_P28482-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2234967

Product Specific Information

PA1-9591 detects Phospho-Erk1/2 MAP Kinase in human, mouse and rat samples.

PA1-9591 has been successfully used in Western blot, immunohistochemistry, and immunocytochemistry procedures. By Western blot, PA1-9591 detects a 42 kDa and 44 kDa protein representing Phospho-Erk1/2 MAP Kinase.

PA1-9591 immunogen is a phosphorylated peptide (pT202-E-pY204) based on human Erk1/2 MAP Kinase

Target Information

ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.