Thermo Fisher Scientific Phospho-p38 MAPK (Thr180, Tyr182) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (IHC)

1:200 - 1:500

-

Immunocytochemistry (ICC/IF)

1:200 - 1:500

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Phosphorylated peptide (pT180-G-pY182) based on human p38 MAP Kinase

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2139806

Product Specific Information

PA1-9592 detects Phospho-p38 MAP Kinase in human, mouse and rat samples.

PA1-9592 has been successfully used in Western blot, immunohistochemistry, and immunocytochemistry procedures. By Western blot, PA1-9592 detects a 43 kDa protein representing Phospho-p38 MAP Kinase.

PA1-9592 immunogen is a phosphorylated peptide (pT180-G-pY182) based on human p38 MAP Kinase

Target Information

MAPK14 (p38 alpha) is a member of the stress-activated protein kinase class of MAPKs. The p38 MAPK cascade regulates a variety of cellular responses to stress, inflammation, and other signals. p38 MAPK is relatively inactive in the nonphosphorylated form and becomes rapidly activated by dual phosphorylation of a Thr-Gly-Tyr motifs. There are four isoforms of p38 MAPK, which differ in their tissue expression and affinity for upstream activators and downstream effectors. When cells are exposed to tumor necrosis factor, interleukin-1, heat shock, or other activating stimuli, activation of MAPK kinase-3 occurs by phosphorylation. Activated MAPK kinase-3/6 phosphorylate each residue of Thr180 and Tyr182 in p38 MAPK. Phospho-p38 MAPK activates ATF-2, CHOP-1, MEF-2 and other transcription factors through phosphorylation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.