Thermo Fisher Scientific Phospho-p38 MAPK (Thr180, Tyr182) Polyclonal Antibody

Thermo Fisher Scientific Phospho-p38 MAPK (Thr180, Tyr182) Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

PA1-9592 detects Phospho-p38 MAP Kinase in human, mouse, and rat samples.
It has been successfully used in Western blot, immunohistochemistry, and immunocytochemistry procedures.
By Western blot, PA1-9592 detects a 43 kDa protein representing Phospho-p38 MAP Kinase.
The immunogen is a phosphorylated peptide (pT180-G-pY182) based on human p38 MAP Kinase.

Target Information

MAPK14 (p38 alpha) is a member of the stress-activated protein kinase class of MAPKs.
The p38 MAPK cascade regulates various cellular responses to stress, inflammation, and other signals.
p38 MAPK is relatively inactive in its nonphosphorylated form and becomes rapidly activated by dual phosphorylation of a Thr-Gly-Tyr motif.
There are four isoforms of p38 MAPK, differing in tissue expression and affinity for upstream activators and downstream effectors.
Upon exposure to tumor necrosis factor, interleukin-1, heat shock, or other stimuli, MAPK kinase-3 is activated by phosphorylation.
Activated MAPK kinase-3/6 phosphorylates Thr180 and Tyr182 residues in p38 MAPK.
Phospho-p38 MAPK activates transcription factors such as ATF-2, CHOP-1, and MEF-2 through phosphorylation.

Usage Note

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.