Thermo Fisher Scientific Alpha Fetoprotein (AFP) Monoclonal Antibody (ABM334)
인간 AFP 단백질을 인식하는 Mouse IgG1 단일클론 항체로, WB, IHC, ICC/IF, ELISA 등 다양한 분석에 사용 가능. 고순도 Protein A 정제, 안정화 PBS 버퍼 포함. 간암 및 생식세포종 연구용 양성 대조군으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| ELISA | 0.01–0.1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ABM334 |
| Immunogen | Affinity purified human alpha-fetoprotein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Human moderately differentiated hepatocellular carcinoma and human poorly differentiated hepatocellular carcinoma tissues
- Cellular location: Secreted
Target Information
Alpha Fetoprotein (AFP) is a major plasma protein produced by the yolk sac, intestinal tract, and liver during fetal life. In adults, AFP expression is often associated with hepatoma or teratoma, though hereditary persistence may occur without pathology. AFP is considered the fetal counterpart of serum albumin, with both genes located tandemly on chromosome 4. AFP exists in monomeric, dimeric, and trimeric forms and can bind copper, nickel, fatty acids, and bilirubin. AFP levels in amniotic fluid are used to detect renal protein loss and screen for spina bifida and anencephaly. Elevated serum AFP is found in hepatocellular carcinoma, teratoblastoma, colorectal cancer, pancreatic cancer, and germ cell neoplasms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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