Thermo Fisher Scientific F(ab`)2-Goat anti-Human IgG Fc Cross-Adsorbed Secondary Antibody, Biotin
인간 IgG Fc에 특이적인 염소 유래 F(ab')2 단편 2차 항체로, Biotin으로 결합되어 다양한 검출 시스템에 활용 가능. ICC, Flow, ELISA 등에서 높은 민감도와 특이성 제공. 교차흡착 처리로 비특이적 반응 최소화. 연구용 전용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 1:500–1:20,000 |
| Flow Cytometry (Flow) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobulin Fc region |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535949 |
| Target | IgG Fc |
| Cross Adsorption | Against bovine, horse, mouse, and rabbit serum proteins |
| Antibody Form | F(ab')2 Fragment |
Additional Formats Available
- AP
- DyLight 488
- DyLight 550
- DyLight 594
- DyLight 650
- DyLight 680
- DyLight 755
- DyLight 800
- FITC
- HRP
- TRITC
- Custom conjugation available upon request
Target Information
Anti-Human secondary antibodies are affinity-purified with well-characterized specificity for human immunoglobulins. They are widely used for detection, sorting, or purification of specific targets. Secondary antibodies enhance versatility by allowing the use of various detection systems (e.g., HRP, AP, fluorescence) and provide greater sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody. Typically, these antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species, followed by purification and modification (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to yield highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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