
Thermo Fisher Scientific F(ab`)2-Goat anti-Human IgG Fc Cross-Adsorbed Secondary Antibody, HRP
HRP 결합 F(ab')2 염소 항-인간 IgG Fc 2차 항체로, Western blot, IHC, ELISA에 적합합니다. 다양한 종단백질에 교차흡착 처리되어 비특이적 결합이 최소화되었습니다. 동결건조 형태로 제공되며, 높은 순도의 친화 크로마토그래피 정제 제품입니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobulin Fc region |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.1% ProClin 150 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2535945 |
| Target | IgG Fc |
| Cross Adsorption | Against bovine, horse, mouse, and rabbit serum proteins |
| Antibody Form | F(ab')2 Fragment |
Product Specific Information
Rehydrate with 0.55 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.)
Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Store lyophilized material at 2–8°C. For long-term storage after reconstitution, dilute with 50% glycerol and store at -20°C as a liquid.
Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin human serum proteins, light chains on all human immunoglobulins, human IgG F(ab')2 fragment, or serum proteins from bovine, horse, mouse, or rabbit.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins.
They are used for detection, sorting, or purification of human immunoglobulin targets.
Secondary antibodies offer increased versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
They are typically generated by immunizing host animals with pooled immunoglobulins from the target species and can be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) for high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Available Conjugate Formats
- AP
- Biotin
- DyLight 488
- DyLight 550
- DyLight 594
- DyLight 650
- DyLight 680
- DyLight 755
- DyLight 800
- FITC
- TRITC
- Custom conjugation available upon request
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