
Thermo Fisher Scientific F(ab`)2-Goat anti-Human IgG Fc Cross-Adsorbed Secondary Antibody, TRITC
TRITC로 결합된 염소 유래 항-인간 IgG Fc 2차 항체로, 면역세포염색(ICC/IF)에 적합합니다. F(ab')2 단편 형태로 비특이적 결합을 최소화하며, 다양한 종 단백질에 교차흡수 처리되었습니다. 552/578 nm 형광 특성을 가지며 연구용으로 사용됩니다.
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Applications
- Immunocytochemistry (ICC/IF): 1:500–1:20,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobulin Fc region |
| Conjugate | TRITC |
| Excitation/Emission Max | 552 / 578 nm |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. Store in the dark. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535950 |
| Target | IgG Fc |
| Cross Adsorption | Against bovine, horse, mouse, and rabbit serum proteins |
| Antibody Form | F(ab`)2 Fragment |
Product Specific Information
- Rehydrate with 0.55 mL of deionized water and let stand for 30 minutes at room temperature to dissolve.
- Centrifuge to remove particulates. Prepare fresh working dilution daily.
- Shelf life: 1 year from date of receipt.
- Based on immunoelectrophoresis, no reactivity observed to: non-immunoglobulin human serum proteins, light chains on all human immunoglobulins, human IgG F(ab')2 fragment, and serum proteins from bovine, horse, mouse, or rabbit.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins.
They are useful for detection, sorting, or purification of target antibodies. Secondary antibodies enhance versatility and sensitivity by enabling multiple detection systems (e.g., HRP, AP, fluorescence) and signal amplification.
Typically, these antibodies are generated by immunizing host animals with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, or conjugation) for high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Additional Formats Available
- AP
- Biotin
- DyLight 488 / 550 / 594 / 650 / 680 / 755 / 800
- FITC
- HRP
- Custom conjugation available upon request
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