Thermo Fisher Scientific F(ab`)2-Goat anti-Human IgM (Heavy chain) Secondary Antibody
Human IgM(Heavy chain)에 특이적인 염소 유래 F(ab')2 2차항체로, Western blot, ICC/IF, ELISA 등에 사용 가능. 비결합(Unconjugated) 형태이며, 고순도 친화 크로마토그래피로 정제됨. ELISA와 IEP로 로트별 민감도 및 특이성 검증 완료.
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Applications and Tested Dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | 1:2000 |
| Immunocytochemistry (ICC/IF) | 1:250 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Human Mu immunoglobulin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535953 |
| Target | IgM (Heavy chain) |
| Antibody Form | F(ab`)2 Fragment |
Product Specific Information
Each lot’s sensitivity is confirmed using ELISA, and specificity is validated by immunoelectrophoresis (IEP).
Target Information
Anti-Human secondary antibodies are affinity-purified reagents with well-characterized specificity for human immunoglobulins. They are useful for detection, sorting, or purification of target antibodies.
Secondary antibodies enhance versatility and sensitivity by allowing multiple detection systems (e.g., HRP, AP, fluorescence) and signal amplification.
Typically, these antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and can be further purified or modified (e.g., immunoaffinity chromatography, fragmentation, label conjugation) for high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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