Thermo Fisher Scientific F(ab`)2-Goat anti-Human IgG Fc Cross-Adsorbed Secondary Antibody, FITC
FITC 결합된 염소 유래 항-인간 IgG Fc 단편 항체로, ICC/IF 및 유세포분석에 적합합니다. F(ab')₂ 형태로 비특이적 결합을 최소화하며, 광범위한 교차흡착 처리로 높은 특이성과 신뢰성을 제공합니다. 연구용으로만 사용됩니다.
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- A24477
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Thermo Fisher Scientific F(ab`)2-Goat anti-Human IgG Fc Cross-Adsorbed Secondary Antibody, FITC
Applications and Tested Dilution
- Immunocytochemistry (ICC/IF): 1:500–1:20,000
- Flow Cytometry (Flow): 1:500–1:20,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobulin Fc region |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.8, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. Store in the dark. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535946 |
| Target | IgG Fc |
| Cross Adsorption | Against bovine, horse, mouse, and rabbit serum proteins |
| Antibody Form | F(ab`)2 Fragment |
Available Conjugate Formats
- AP
- Biotin
- DyLight 488, 550, 594, 650, 680, 755, 800
- HRP
- TRITC
- Custom conjugation available upon request
Target Information
Anti-Human secondary antibodies are affinity-purified and exhibit well-characterized specificity for human immunoglobulins. They are useful for detection, sorting, or purification of target proteins.
Secondary antibodies enhance versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically generated by immunizing host animals with pooled immunoglobulins from the target species, these antibodies may be further purified and modified (e.g., immunoaffinity chromatography, fragmentation, label conjugation) to yield highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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