Thermo Fisher Scientific Importin 9 Polyclonal Antibody
Importin 9 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, Flow Cytometry에 사용 가능. Human 및 Mouse 반응성. 고순도 Protein A 및 Antigen affinity chromatography 정제. 연구용 핵단백질 수송 관련 연구에 적합.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 | - |
| Flow Cytometry (Flow) | 1:10–1:50 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide between 79–106 amino acids from the N-terminal region of human IPO9 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A, Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2542977 |
Target Information
Functions in nuclear protein import as a nuclear transport receptor. Serves as a receptor for nuclear localization signals (NLS) in cargo substrates. Mediates docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin, followed by translocation through the pore via an energy-dependent, Ran-mediated mechanism.
At the nucleoplasmic side of the NPC, Ran binding causes dissociation of the importin/substrate complex, and importin is re-exported to the cytoplasm where GTP hydrolysis releases Ran.
This directionality of nuclear import is conferred by asymmetric distribution of GTP- and GDP-bound forms of Ran between cytoplasm and nucleus.
Importin 9 mediates nuclear import of H2B histone, RPS7, and RPL18A, prevents cytoplasmic aggregation of RPS7 and RPL18A, and may also import H2A, H3, H4 histones, RPL4, and RPL6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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