Thermo Fisher Scientific PSME4 Polyclonal Antibody
PA200 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, ICC/IF, IP 등에 사용 가능. Bovine, Human, Mouse, Rat 시료에서 반응하며, 핵 내 PA200 발현 연구에 적합. 합성 펩타이드(AA 1620–1634)를 면역원으로 제작됨.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:250 | View 3 publications |
| Immunocytochemistry (ICC/IF) | 1:600 | View 1 publication |
| Immunoprecipitation (IP) | Assay-dependent | – |
| ChIP assay (ChIP) | – | View 1 publication |
| Miscellaneous PubMed (Misc) | – | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to residues T(1620) K L P K K R K R D P G S V G(1634) of human PA200 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Whole serum |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2171425 |
Product Specific Information
PA1-1961 detects PA200 from bovine, human, mouse, and rat samples.
It has been successfully used in Western blot, immunofluorescence, and immunoprecipitation assays.
- Western blot: Detects ~200 kDa PA200 protein in bovine testis extract. In mouse tissues, detects both 200 kDa (testis-enriched) and 160 kDa (spleen-enriched) proteins.
- Immunofluorescence: In HeLa cells, PA200 shows diffuse nuclear staining before ionizing radiation, and focal nuclear aggregates after treatment.
- Immunogen: Synthetic peptide corresponding to residues T(1620)–G(1634) of human PA200.
Target Information
PA200 (PSME4) is an associated proteasome component that recognizes acetylated histones and promotes ATP- and ubiquitin-independent degradation of core histones during spermatogenesis and DNA damage response.
It binds acetylated histones via its bromodomain-like region and activates the proteasome by opening the gated channel for substrate entry.
As part of the spermatoproteasome, PA200 promotes histone degradation and exchange during spermatogenesis and contributes to DNA damage response by facilitating histone degradation following double-strand breaks.
[UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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