Thermo Fisher Scientific PSME4 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:250

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:600

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

-

ChIP assay (ChIP)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues T(1620) K L P K K R K R D P G S V G(1634) of human PA200. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PSME4,uniProtId:Q14997-1,ncbiNodeId:9606,antigenRange:1620-1634,antigenLength:1843,antigenImageFileName:PA1-1961_PSME4_Q14997-1_Rabbit.svg,antigenImageFileNamePDP:PA1-1961_PSME4_Q14997-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2171425

Product Specific Information

PA1-1961 detects PA200 from bovine, human, mouse and rat samples.

PA1-1961 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects an ~200 kDa protein representing PA200 from bovine testis tissue extract. In mouse tissues, this antibody detects both a 200 kDa and a 160 kDa protein. In mouse tissues, the 200 kDa species is most abundant in testis whereas the 160 kDa species is enriched in the spleen. Immunofluorescence staining of PA200 in HeLa cells results in diffuse nuclear staining prior to ionizing radiation treatment. Immunofluorescence staining of PA200 in HeLa cells after ionizing radiation treatment results in focal aggregate staining within the nucleus.

The PA1-1961 immunogen is a synthetic peptide corresponding to residues T(1620) K L P K K R K R D P G S V G(1634) of human PA200.

Target Information

Associated component of the proteasome that specifically recognizes acetylated histones and promotes ATP- and ubiquitin-independent degradation of core histones during spermatogenesis and DNA damage response. Recognizes and binds acetylated histones via its bromodomain-like (BRDL) region and activates the proteasome by opening the gated channel for substrate entry. Binds to the core proteasome via its C-terminus, which occupies the same binding sites as the proteasomal ATPases, opening the closed structure of the proteasome via an active gating mechanism. Component of the spermatoproteasome, a form of the proteasome specifically found in testis: binds to acetylated histones and promotes degradation of histones, thereby participating actively to the exchange of histones during spermatogenesis. Also involved in DNA damage response in somatic cells, by promoting degradation of histones following DNA double-strand breaks. [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.