Thermo Fisher Scientific PSMD7 Polyclonal Antibody

Applications

Product Specifications

Product Specific Information

PA1-1963 detects proteasome 19S subunit S12 from human, rat, mouse, and hamster cell lysates.
It has been successfully used in Western blot procedures, detecting an ~37 kDa protein representing proteasome 19S subunit S12. An additional unknown band (~48 kDa) may appear in some lysates.

The immunogen is a synthetic peptide corresponding to residues A(55) V P F D E D D K D D S(66) C of human 19S subunit S12.
The immunizing peptide sequence is 100% conserved in Danio rerio and 91% conserved in Drosophila, C. elegans, and Anopheles.
PA1-1963 immunizing peptide (Cat. # PEP-211) is available for use in neutralization and control experiments.

Target Information

Proteolytic degradation maintains appropriate levels of short-lived and regulatory proteins involved in metabolism, stress response, antigen presentation, receptor modulation, cell cycle, transcription, and signaling.
The ubiquitin-proteasome pathway deconstructs most cytosolic and nuclear proteins, while others are degraded via the vacuolar pathway (endosomes, lysosomes, ER).

The 26S proteasome is an ATP-dependent, multisubunit (~31) molecular machine (~2.5 MDa) composed of a 20S core complex and one or two 19S regulatory subunits.
These 19S subunits recognize ubiquitinated proteins and facilitate their unfolding and translocation into the 20S lumen.
Defects in this pathway are linked to diseases such as cystic fibrosis, Angelman’s syndrome, and Liddle syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.