Thermo Fisher Scientific MTCO2 Monoclonal Antibody (12C4F12)

Applications and Tested Dilution

Product Specifications

Product Specific Information

To facilitate the study of COX structure and mitochondrial biogenesis, Invitrogen offers several subunit-specific mouse anti-OxPhos Complex IV monoclonal antibodies.
The binding specificity of these antibodies allows researchers to investigate the regulation, assembly, and orientation of COX subunits in various organisms.

These antibodies are valuable for analyzing human mitochondrial myopathies and related disorders, as well as mitochondrial DNA depletion due to drug toxicity.
Alexa Fluor™ 488 and Alexa Fluor™ 594 conjugates of anti-OxPhos Complex IV subunit I are also available for direct staining.

Cell lines can be screened for subunit expression levels by Western blotting, and results can be complemented with native gel electrophoresis or sucrose gradient centrifugation to assess complex assembly.

Many OxPhos subunit antibodies are suitable for immunocytochemical analysis, aiding in the visualization of subunit localization and expression, particularly in diseased muscle fibers or Complex IV-deficient samples.

The antibody was produced in vitro using hybridomas grown in serum-free medium and purified by biochemical fractionation. Purity was verified by SDS-PAGE.

Target Information

Cytochrome c oxidase (Complex IV) is the terminal enzyme of the mitochondrial electron transport chain driving oxidative phosphorylation.
It catalyzes the reduction of oxygen to water using electrons from cytochrome c and protons from the mitochondrial matrix.

The respiratory chain includes three multisubunit complexes:

• Complex II (succinate dehydrogenase, CII)
• Complex III (ubiquinol-cytochrome c oxidoreductase, CIII)
• Complex IV (cytochrome c oxidase, CIV)

These complexes cooperate to transfer electrons from NADH and succinate to molecular oxygen, generating the electrochemical gradient that drives ATP synthesis.

Within Complex IV, electrons are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the binuclear center (BNC) formed by heme A3 and copper B (CU(B)), which reduces oxygen to water.
[UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.