Thermo Fisher Scientific MTCO2 Monoclonal Antibody (12C4F12)
MTCO2 단클론 항체(12C4F12)는 인간 미토콘드리아 복합체 IV의 구성요소를 인식하는 항체로, Western blot과 면역형광 분석에 적합합니다. COX 구조 및 미토콘드리아 생합성 연구에 유용하며, 인간 미토콘드리아 근병증 및 관련 질환 연구에 활용됩니다.
- 카탈로그번호
- A6404
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific MTCO2 Monoclonal Antibody (12C4F12)
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | View 68 publications |
| Immunohistochemistry (IHC) | – | View 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | – | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | – | View 1 publication |
| Immunocytochemistry (ICC/IF) | 5–10 µg/mL | View 7 publications |
| Miscellaneous PubMed (Misc) | – | View 6 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Chicken, Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 12C4F12 |
| Immunogen | Full-length native Human Cytochrome C oxidase (Complex IV) subunit II (MTCO2, UniProt ID: P00403-1, amino acids 1–227) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | IgG fraction |
| Storage Buffer | HEPES buffered saline |
| Contains | 0.02% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Wet ice |
| RRID | AB_221584 |
Product Specific Information
To facilitate the study of COX structure and mitochondrial biogenesis, Invitrogen offers several subunit-specific mouse anti-OxPhos Complex IV monoclonal antibodies.
The binding specificity of these antibodies allows researchers to investigate the regulation, assembly, and orientation of COX subunits in various organisms.
These antibodies are valuable for analyzing human mitochondrial myopathies and related disorders, as well as mitochondrial DNA depletion due to drug toxicity.
Alexa Fluor™ 488 and Alexa Fluor™ 594 conjugates of anti-OxPhos Complex IV subunit I are also available for direct staining.
Cell lines can be screened for subunit expression levels by Western blotting, and results can be complemented with native gel electrophoresis or sucrose gradient centrifugation to assess complex assembly.
Many OxPhos subunit antibodies are suitable for immunocytochemical analysis, aiding in the visualization of subunit localization and expression, particularly in diseased muscle fibers or Complex IV-deficient samples.
The antibody was produced in vitro using hybridomas grown in serum-free medium and purified by biochemical fractionation. Purity was verified by SDS-PAGE.
Target Information
Cytochrome c oxidase (Complex IV) is the terminal enzyme of the mitochondrial electron transport chain driving oxidative phosphorylation.
It catalyzes the reduction of oxygen to water using electrons from cytochrome c and protons from the mitochondrial matrix.
The respiratory chain includes three multisubunit complexes:
- Complex II (succinate dehydrogenase, CII)
- Complex III (ubiquinol-cytochrome c oxidoreductase, CIII)
- Complex IV (cytochrome c oxidase, CIV)
These complexes cooperate to transfer electrons from NADH and succinate to molecular oxygen, generating the electrochemical gradient that drives ATP synthesis.
Within Complex IV, electrons are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the binuclear center (BNC) formed by heme A3 and copper B (CU(B)), which reduces oxygen to water.
[UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.