Thermo Fisher Scientific CD16 Monoclonal Antibody (eBioCB16 (CB16)), eFluor 450, eBioscience
CD16(FcγRIII)를 인식하는 eBioCB16 단클론 항체로, 인간 세포 분석용. eFluor 450 형광체 결합으로 405 nm 레이저에 적합. NK세포 및 대식세포 표지에 활용. Flow cytometry에 최적화된 프리타이틀 제품.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 5 µL (0.25 µg)/test | View 26 publications |
| T-Cell Activation (TCA) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 450, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioCB16 (CB16) |
| Conjugate | eFluor™ 450 |
| Excitation/Emission Max | 405/445 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1272122 |
Available Formats
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 661
- Brilliant UV 737
- Brilliant UV 805
- Brilliant Violet 480
- eFluor 506
- FITC
- Functional Grade
- NovaFluor Blue 610-70S
- NovaFluor Blue 660-120S
- NovaFluor Red 710
- NovaFluor Red 725
- NovaFluor Yellow 660
- NovaFluor Yellow 730
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Super Bright 780
- Request custom conjugation
Product Specific Information
The eBioCB16 monoclonal antibody recognizes CD16 (Fc gammaRIII), a low-affinity receptor for IgG (50–80 kDa).
CD16 includes two gene variants:
- CD16A (FcγRIIIA): Expressed on macrophages and NK cells, transmembrane-anchored.
- CD16B (FcγRIIIB): Expressed on neutrophils, GPI-anchored form.
Two polymorphisms (NA-1 and NA-2) exist; NA-2 homozygotes show reduced phagocytic capacity.
CD16 binds IgG immune complexes, with preference for IgG1 and IgG3, and mediates ADCC, phagocytosis, degranulation, and proliferation.
Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated for flow cytometry on normal human peripheral blood cells at 5 µL (0.25 µg)/test.
A test is defined as the amount of antibody to stain 10⁵–10⁸ cells in 100 µL volume.
Fluorophore: eFluor® 450
- Excitation: 405 nm
- Emission: 445 nm
- Laser: Violet
- Alternative to Pacific Blue®
- 0.2 µm filtered after manufacturing
Target Information
CD16 (FCGR3A) encodes a receptor recognizing the Fc portion of IgG, involved in immune complex clearance, cytotoxicity, and viral infection enhancement.
FCGR3A and FCGR3B share high similarity and are located on chromosome 1.
- FCGR3A: Expressed on NK cells, transmembrane-anchored.
- FCGR3B: Expressed on neutrophils, PI-anchored.
Mutations in FCGR3A are linked to Immunodeficiency 20, Herpes Zoster, and Systemic Lupus Erythematosus susceptibility.
Alternative splicing produces multiple isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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