Thermo Fisher Scientific CD14 Monoclonal Antibody (61D3), eFluor 450, eBioscience
인간 CD14 단백질을 인식하는 61D3 단클론 항체로, eFluor 450 형광체로 표지되어 있습니다. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 405 nm 레이저로 여기되어 445 nm에서 방출됩니다. 단핵구 및 대식세포 표면 CD14 검출에 적합합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunocytochemistry (ICC/IF) | - | View 4 publications |
| Flow Cytometry (Flow) | 5 µL (0.5 µg)/test | View 61 publications |
| Neutralization (Neu) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 450 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 61D3 |
| Conjugate | eFluor™ 450 |
| Excitation / Emission Max | 405 / 445 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1272120 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395, 496, 615, 661, 737, 805
- Brilliant Violet 421, 480, 605, 650, 711, 786
- eFluor 506, FITC, Functional Grade
- NovaFluor Blue 510, 555, 585, 610-30S, 660-40S, 800
- NovaFluor Yellow 570, 660
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Request custom conjugation available
Product Specific Information
The 61D3 monoclonal antibody reacts with human CD14, a 53–55 kDa GPI-linked glycoprotein expressed on monocytes, interfollicular macrophages, and some dendritic cells. Complexes of LPS and LBP bind with high affinity to monocytes through surface CD14.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-titrated and validated for flow cytometry using normal human peripheral blood cells. Recommended use: 5 µL (0.5 µg) per test (100 µL final volume).
- Fluorophore Information: eFluor® 450 is excited at 405 nm and emits at 445 nm, compatible with violet laser detection.
- Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD14 is a 55 kDa GPI-anchored glycoprotein constitutively expressed on mature monocytes, macrophages, and neutrophils. It functions as a lipopolysaccharide receptor and exists in both membrane-bound and soluble forms. CD14 binds LPS in a reaction catalyzed by LBP and plays roles in immune recognition, inflammation, and apoptotic cell clearance. Dysregulation of CD14 is associated with infections and inflammatory diseases such as Croup and mycobacterium chelonae infection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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