Thermo Fisher Scientific CD11c Monoclonal Antibody (3.9), eFluor 450, eBioscience
인간 CD11c 단백질을 인식하는 단클론 항체로, eFluor 450 형광체가 결합되어 있습니다. 유세포 분석(Flow Cytometry)에 최적화되어 있으며, 405 nm 레이저로 여기되어 445 nm에서 방출됩니다. 단핵구, 대식세포, 수지상세포 등에서 CD11c 검출에 사용됩니다.
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Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 5 µL (0.5 µg)/test
- Publications: View 19 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 450, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3.9 |
| Conjugate | eFluor™ 450 |
| Excitation / Emission Max | 405 / 445 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11218498 |
Available Formats
- Unconjugated
- Alexa Fluor 532
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 737
- Brilliant UV 805
- eFluor 506
- FITC
- PE
- PE-Cyanine5
- PE-Cyanine5.5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Super Bright 780
- Request custom conjugation
Product Specific Information
Description:
The 3.9 monoclonal antibody reacts with human CD11c, a 150 kDa integrin alpha X chain. CD11c associates with beta 2 integrin to form the CD11c/CD18 heterodimer, expressed on monocytes, granulocytes, macrophages, NK cells, dendritic cells, and subsets of T and B lymphocytes. This complex binds to CD54, iC3b, and fibrinogen, playing a key role in leukocyte adhesion.
Applications Reported:
Reported for use in flow cytometric analysis.
Applications Tested:
Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. Recommended at 5 µL (0.5 µg) per test (final volume 100 µL). Cell number can range from 10⁵ to 10⁸ cells/test.
Fluorophore Information:
eFluor® 450 is an alternative to Pacific Blue®, excited at 405 nm and emitting at 445 nm (Violet laser). Ensure instrument compatibility.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD11, together with CD18, forms a heterodimeric adhesion molecule.
- CD11a: Expressed in B/T lymphocytes, macrophages, monocytes, neutrophils, basophils, eosinophils.
- CD11b: Expressed by monocytes, macrophages, NK cells, some B/T cells, and granulocytes.
- CD11c: Expressed in monocytes, macrophages, NK cells, some granulocytes, and subsets of lymphocytes.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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