Thermo Fisher Scientific Estrogen Receptor alpha Monoclonal Antibody (AER311)
에스트로겐 수용체 알파(ERα)를 인식하는 단클론 항체로, Western blot, IHC, ICC/IF, ELISA, IP, ChIP 등 다양한 응용에 적합합니다. Mouse IgG2a 형식이며, 인간, 마우스, 소 시료에 반응합니다. 1 mg/mL 농도의 액상 제품으로 -20°C에서 보관합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 7 publications |
| Immunohistochemistry (IHC) | Assay-dependent | View 5 publications |
| Immunocytochemistry (ICC/IF) | 1:100 | View 1 publication |
| ELISA | Assay-dependent | - |
| Immunoprecipitation (IP) | Assay-dependent | - |
| ChIP assay (ChIP) | 2.5 µg/10^6 cells | - |
| Gel Shift (GS) | - | View 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Bovine, Human, Mouse |
| Published Species | Bovine, Human, Mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | AER311 |
| Immunogen | Estrogen receptors from calf uterus |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | PBS |
| Contains | 0.08% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1074280 |
Target Information
Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor that, when bound to estrogen, undergoes a conformational change enabling dimerization and binding to estrogen response elements (ERE) in DNA. This interaction regulates gene transcription through coactivator or corepressor recruitment.
There are two main forms of estrogen receptors, alpha (ESR1) and beta (ESR2), encoded by separate genes. Due to alternative RNA splicing, at least four estrogen receptor-alpha isoforms exist (66 kDa, 53 kDa, 47 kDa, 35 kDa). Estrogen receptors are widely expressed and are essential for sexual development, reproductive function, and bone maintenance. They are also implicated in diseases such as breast cancer, endometrial cancer, and osteoporosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송/결제/교환/반품 안내
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