Thermo Fisher Scientific PD-L2 (PDCD1LG2) Monoclonal Antibody (OTI6H10), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:500

Flow Cytometry (Flow)

• Tested Dilution: Assay-Dependent

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of about 1 mg/mL.
For conjugation experiments, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

Programmed death-ligand 2 (PD-L2), also known as B7-DC, is a member of the B7 ligand family within the immunoglobulin superfamily. PD-L2 acts as a ligand for PD-1, similar to PD-L1, and is primarily expressed in dendritic cells. Its expression can be induced in various immune and non-immune cells depending on the microenvironment, particularly by cytokines such as IL-4, TNF-α, and IFN-γ.

PD-L2 binds PD-1 with higher affinity than PD-L1, functioning as an inhibitory immune checkpoint molecule that suppresses T cell activation, proliferation, and cytokine secretion (e.g., IFN-γ, IL-10). It can also stimulate T cell proliferation in PD-1-deficient cells, indicating additional receptors such as RGMb. The PD-L2–RGMb interaction regulates respiratory tolerance through BMP and/or neogenin signaling pathways.

The human PD-L2 monomer comprises:

• 201-amino-acid extracellular domain
• 21-amino-acid transmembrane domain
• 32-amino-acid cytoplasmic domain

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.