Thermo Fisher Scientific CD200 Monoclonal Antibody (OX104), PE, eBioscience

Applications

Tested Application

• Flow Cytometry (Flow)

Tested Dilution

• 5 µL (0.25 µg)/test

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Product Specifications

Additional Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant Violet 711
• eFluor 450
• FITC
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 710
• NovaFluor Yellow 610
• NovaFluor Yellow 700
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

Description:
The monoclonal antibody OX104 recognizes human CD200 (OX2), a member of the immunoglobulin superfamily with two Ig domains, a transmembrane and cytoplasmic domain. CD200 is expressed on resting and activated B cells, a subset of T cells, keratinocytes, peripheral and central nerve cells, follicular dendritic cells, and ovarian cells. Interaction with CD200R leads to macrophage activation (IL-6 production), inhibition of mast cell degranulation, reduced TNF-α and IL-13 secretion, and attenuation of lymphocyte activation. It may also play a role in maternal tolerance due to trophoblast expression.

Applications Reported:

• Flow cytometric analysis

Applications Tested:

• Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
• Recommended use: 5 µL (0.25 µg) per test
• A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL
• Cell number range: 10⁵–10⁸ cells/test

Excitation/Emission:

• Excitation: 488–561 nm
• Emission: 578 nm
• Compatible Lasers: Blue, Green, Yellow-Green

Filtration:

• 0.2 µm post-manufacturing filtered

Target Information

CD200 is a type-1 membrane glycoprotein with two immunoglobulin domains, belonging to the immunoglobulin superfamily. It is a broadly distributed cell surface glycoprotein interacting with CD200R on myeloid cells, preventing macrophage activation. Homologues exist in herpesviruses and poxviruses. Studies in mouse and rat suggest CD200 regulates myeloid cell activity and delivers inhibitory signals to macrophages. Multiple alternatively spliced transcript variants exist. CD200 dysfunction is associated with Mantle Cell Lymphoma and Primary Hypertrophic Osteoarthropathy.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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