Thermo Fisher Scientific CD11c Monoclonal Antibody (N418), Alexa Fluor 660, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 700
• APC, APC-eFluor 780
• Biotin, Brilliant UV 395–805, Brilliant Violet 421–711
• eFluor 450, 506, 615, 660
• FITC, PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
• PE-eFluor 610, PerCP-Cyanine5.5
• Super Bright 436, 600, 645, 780
• NovaFluor Blue 510–690, NovaFluor Yellow 570
• Functional Grade
• Request custom conjugation

Product Specific Information

The N418 monoclonal antibody reacts with mouse CD11c (integrin alpha X). CD11c non-covalently associates with beta2 integrin to form the CD11c/CD18 heterodimer. CD11c is expressed by dendritic cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. It binds to CD54, iC3b, and fibrinogen, playing a role in leukocyte adhesion.
N418 binds CD11c on splenic dendritic cells in T-dependent spleen areas and precipitates a 150, 90 kDa heterodimer.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Flow cytometric analysis of mouse splenocytes; ≤0.5 µg/test
• Recommended Use: Optimize titration for best assay performance
• Fluorophore: Alexa Fluor 660 (Emits at 690 nm; Excitation 662 nm; Laser: Red Laser)
• Suitable for use on spectral cytometers, multiplexed with APC, Alexa Fluor 647, and Alexa Fluor 700.

Target Information

CD11 and CD18 form a heterodimer adhesion molecule.

• CD11a: Expressed in B/T lymphocytes, macrophages, monocytes, neutrophils, basophils, eosinophils
• CD11b: Expressed in monocytes, macrophages, NK cells, some B/T cells, granulocytes
• CD11c: Expressed in monocytes, macrophages, NK cells, some granulocytes, and subsets of lymphocytes

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.