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Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), Alexa Fluor 660, eBioscience
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Thermo Fisher Scientific

Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), Alexa Fluor 660, eBioscience

상품 한눈에 보기

Mouse CD11b 검출용 Alexa Fluor 660 결합 단클론 항체로, 유세포 분석에 최적화되어 있습니다. Mac-1 복합체 인식 및 인간 CD11b에도 교차 반응합니다. 고순도 친화 크로마토그래피 정제 제품으로, 4°C 암저장 조건에서 안정적입니다.

브랜드
Thermo Fisher Scientific
카테고리
Monoclonal Antibodies
판매단위
pk

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Thermo Fisher Scientific 606-0112-80 CD11b Monoclonal Antibody (M1/70), Alexa Fluor 660, eBioscience 25 ug pk판매 단위 pk
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Thermo Fisher Scientific · Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), Alexa Fluor 660, eBioscience

Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), Alexa Fluor 660, eBioscience

Applications

  • Flow Cytometry (Flow)

Tested Dilution: 0.25 µg/test

Product Specifications

항목 내용
Species Reactivity Mouse
Host / Isotype Rat / IgG2b, kappa
Recommended Isotype Control Rat IgG2b kappa Isotype Control (eB149/10H5), Alexa Fluor™ 660, eBioscience™
Class Monoclonal
Type Antibody
Clone M1/70
Conjugate Alexa Fluor™ 660
Excitation / Emission Max 663 / 691 nm
Form Liquid
Concentration 0.2 mg/mL
Purification Affinity chromatography
Storage Buffer PBS, pH 7.2
Contains 0.09% sodium azide
Storage Conditions 4°C, store in dark, DO NOT FREEZE
Shipping Conditions Ambient (domestic); Wet ice (international)
RRID AB_2896244

Additional Formats Available

  • Unconjugated
  • Alexa Fluor 488, 532, 700
  • APC, APC-eFluor 780
  • Biotin
  • Brilliant UV 395–805 Series
  • Brilliant Violet 421–786 Series
  • eFluor 450, 506, 660
  • FITC
  • Functional Grade
  • NovaFluor Blue / Yellow Series
  • PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
  • PerCP-Cyanine5.5, PerCP-eFluor 710
  • Super Bright 436–780 Series
  • Custom conjugation available

Product Specific Information

The M1/70 monoclonal antibody reacts with mouse CD11b, a 165–170 kDa integrin alphaM. CD11b associates with CD18 to form alphaMbeta2 integrin (Mac-1) and binds CD54 (ICAM-1), C3bi, and fibrinogen.
Mac-1 is expressed by macrophages, NK cells, granulocytes, activated lymphocytes, and mouse B-1 cells. M1/70 also cross-reacts with human CD11b, enabling detection on human monocytes, granulocytes, and NK cell subsets.
CD11b participates in adhesive cell interactions through ligand binding.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse bone marrow cells at ≤0.25 µg/test (100 µL final volume). Optimize titration per assay.
Fluorophore: Alexa Fluor 660 (Emits at 690 nm; Excitation 662 nm; Laser: Red)

Target Information

CD11b (integrin alpha-M, ITGAM) forms a heterodimer with CD18 (integrin beta-2), known as Mac-1 or CR3.
Expressed on monocytes/macrophages, granulocytes, activated lymphocytes, NK cells, dendritic cells, and microglia.
Functions as receptor for ICAM-1, ICAM-2, ICAM-4, CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X.
Expression varies by tissue and cell type; used as microglial marker in nervous tissue.
Associated with systemic lupus erythematosus and ITGAM-related susceptibility.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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