Thermo Fisher Scientific TCR beta Monoclonal Antibody (H57-597), Biotin, eBioscience

Applications and Tested Dilution

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Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 496
• Brilliant UV 661
• Brilliant UV 737
• Brilliant Violet 421
• eFluor 450
• eFluor 506
• FITC
• Functional Grade
• NovaFluor Blue 510
• NovaFluor Blue 530
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 725
• NovaFluor Blue 800
• NovaFluor UV 765
• NovaFluor Violet 690
• NovaFluor Violet 800
• NovaFluor Yellow 810
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Super Bright 780
• Request custom conjugation

Product Specific Information

Description:
The H57-597 monoclonal antibody reacts with the beta chain of mouse TCR. TCR beta is expressed by thymocytes in a developmentally regulated manner and by most peripheral T cells. Crosslinking of the TCR complex with H57-597 induces activation and proliferation of T cells or apoptosis depending on assay conditions. H57-597 is used as a phenotypic marker for TCR beta expressing T cells.

Applications Reported:
Reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of mouse thymocytes and splenocytes. Recommended ≤0.5 µg per test (100 µL final volume). Cell number may range from 10⁵–10⁸ cells/test. Antibody should be titrated for optimal performance.

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

T cell receptors (TCRs) discriminate foreign from self-peptides presented by MHC class II molecules, critical for adaptive immune response. Recognition of self-peptides is linked to autoimmune disease, and mutant self-peptides are associated with tumors. Engagement of TCRs by bacterial superantigens can cause toxic shock syndrome. Autoantibodies to V beta segments of TCRs have been isolated from rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE) patients, potentially modulating TH1-mediated inflammation.

Most human T cells express TCR alpha-beta with either CD4 or CD8 (single positive). A small subset lacks both (double negative), which can increase in autoimmune or immunodeficiency disorders. Gamma-delta T cells, found mainly in epithelia, show limited diversity and distinct antigen recognition, with roles in antitumor and immunoregulatory activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.