Thermo Fisher Scientific CD49b (Integrin alpha 2) Monoclonal Antibody (DX5), Biotin, eBioscience
Mouse NK 및 T 세포에서 CD49b 항원을 검출하는 DX5 클론 단일클론 항체. Biotin 결합으로 유세포 분석에 적합. 0.25 µg/test로 사용 가능하며, 4°C 암소 보관. 다양한 마우스 균주에 반응하며 NK1.1과 함께 공동발현 분석 가능.
- 카탈로그번호
- 13-5971-8x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD49b (Integrin alpha 2) Monoclonal Antibody (DX5), Biotin, eBioscience
Thermo Fisher Scientific CD49b (Integrin alpha 2) Monoclonal Antibody (DX5), Biotin, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 1 publication |
| Immunohistochemistry (IHC) | - | 2 publications |
| Flow Cytometry (Flow) | 0.25 µg/test | 29 publications |
| Functional Assay | - | 1 publication |
| Miscellaneous (PubMed) | - | 8 publications |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgM, kappa |
| Recommended Isotype Control | Rat IgM Isotype Control (eBRM), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | DX5 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466825 |
| Filtration | 0.2 µm post-manufacturing filtered |
Available Formats
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- eFluor 450
- eFluor 506
- FITC
- PE
- PE-Cyanine5
- PE-Cyanine5.5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Custom conjugation available upon request
Product Specific Information
The DX5 monoclonal antibody reacts with CD49b, an antigen expressed on a majority of mouse natural killer (NK) cells and a subset of T cells. DX5 recognizes all tested mouse strains including BALB/c, C57BL/6, C3H, CBA, DBA, AKR, SJL, and 129.
Simultaneous staining of C57BL/6 spleen cells with anti-NK1.1 mAb (PK136) and DX5 shows coexpression of both markers by most cells, with small populations of DX5⁺PK136⁻ and DX5⁻PK136⁺ cells.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes; can be used at ≤0.25 µg per test (100 µL final volume). Cell number should be determined empirically (10⁵–10⁸ cells/test). Antibody should be titrated for optimal performance.
Target Information
CD49b (Integrin alpha 2) is a member of the integrin family, forming a heterodimeric complex with CD29 (Integrin beta 1) to create VLA-2 (integrin α2β1). This complex serves as an extracellular receptor for laminin, collagen, and fibronectin, activating intracellular signaling pathways.
It plays roles in VEGF-induced angiogenesis, adhesion, and lymphocyte activation. CD49b is expressed by NK cells, NK-T cells, monocytes, platelets, epithelial cells, and subsets of T and B cells, including CD4⁺ Tr1 regulatory cells co-expressing LAG-3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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