Thermo Fisher Scientific CD161 Monoclonal Antibody (HP-3G10), PerCP-Cyanine5.5, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.5 µg)/test
• Publications: View 10 publications

Product Specifications

Additional Formats Available

• Alexa Fluor 488
• APC
• eFluor 450
• PE
• PE-Cyanine7
• PE-eFluor 610
• Request custom conjugation

Product Specific Information

This HP-3G10 monoclonal antibody reacts with CD161 (NKR-P1A), a member of the C-type lectin superfamily. CD161 is expressed on natural killer (NK) cells and various T cell subsets, including memory/effector CD4⁺, CD8⁺, γδTCR T cells, and a subset of CD3⁺ thymocytes. It is also detected on human Th17 CD4⁺ T cells. The receptor’s function is not fully understood but may have a stimulatory role. Lectin-like transcript-1 (LLT1) has been identified as its ligand.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and tested on normal human peripheral blood cells
• Recommended Usage: 5 µL (0.5 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test)
• Excitation / Emission / Laser: 488 nm / 695 nm / Blue Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

KLRB1 (CD161) is a type II membrane protein expressed on NK cells and a subset of CD3⁺ T cells. It forms a homodimeric structure (40–44 kDa chains) and plays an inhibitory role in NK cell function. Activation of KLRB1 stimulates acid sphingomyelinase/SMPD1, increases intracellular ceramide, and activates AKT1/PKB and RPS6KA1/RSK1 pathways, promoting T cell proliferation. KLRB1 binds to CLEC2D/LLT1 and specific carbohydrate epitopes, modulating NK cell cytotoxicity and interferon-gamma secretion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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