Thermo Fisher Scientific CD158a/h/g Monoclonal Antibody (HP-MA4), PerCP-Cyanine5.5, eBioscience

Thermo Fisher Scientific CD158a/h/g Monoclonal Antibody (HP-MA4), PerCP-Cyanine5.5, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.25 µg)/test
  View 2 publications
• Functional Assay (Functional): -
  View 1 publication

Product Specifications

Additional Formats

• APC
• eFluor 450
• PE
• PE-Cyanine7
• Request custom conjugation

Product Specific Information

The monoclonal antibody HP-MA4 recognizes several CD158 proteins (KIR2D family), specifically KIR2DL1 (CD158a), KIR2DS1 (CD158h), KIR2DS3, and KIR2DS5 (CD158g).
It does not recognize KIR2DL2 (CD158b1), KIR2DS2 (CD158j), KIR2DL4 (CD158d), or KIR2DS4 (CD158i). Crossreactivity to KIR2DL3 (CD158b2) and KIR2DL5 (CD158f) is unknown.

CD158 proteins are members of the killer cell immunoglobulin-like receptors (KIR) family expressed on NK cells and subsets of T cells.
Each KIR has a short (S) or long (L) cytoplasmic domain, mediating activating or inhibitory signals respectively.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and validated on normal human peripheral blood
  • Use at 5 µL (0.25 µg)/test
  • Test = 100 µL final volume (10⁵–10⁸ cells/test)

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect vial and samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.
Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by NK and T cells.
KIR genes are polymorphic and clustered on chromosome 19q13.4 within the leukocyte receptor complex (LRC).
They are classified by the number of extracellular immunoglobulin domains (2D or 3D) and cytoplasmic domain length (L or S).
KIRs with long domains transduce inhibitory signals via ITIM motifs, while short domains associate with TYRO protein tyrosine kinase binding protein for activating signals.
Ligands include subsets of HLA class I molecules, playing a key role in immune regulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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