Thermo Fisher Scientific CD197 (CCR7) Monoclonal Antibody (4B12), PerCP-Cyanine5.5, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 1 µg/test
  View 22 publications

Product Specifications

Additional Available Formats

• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• eFluor 450
• Functional Grade
• PE
• PE-Cyanine7
• PE-eFluor 610
• Request custom conjugation

Product Specific Information

The 4B12 monoclonal antibody reacts with mouse CCR7 (CD197), a chemokine receptor for CCL19 and CCL21. CCR7 mediates the migration of naïve T cells to secondary lymphoid organs and plays a role in dendritic and B cell localization during immune responses.

CCR7 is also a marker for central memory T cells (TCM), characterized by CCR7 and CD62L expression. It is involved in thymocyte development, and its deficiency can lead to aberrant T cell maturation.

Applications

• Reported for use in flow cytometric analysis.
• Tested on mouse splenocytes at ≤1 µg/test.
• Optimal staining achieved at 37°C (brighter signal and better separation).

Recommended Staining Protocol

1. Prepare cell suspension and block FcγR with 5 µg/million cells anti-mouse CD16/32 for 15 min on ice.
2. Without washing, add 1 µg/million cells 4B12 and incubate at 37°C or 2–8°C for 30 min.
3. Wash cells once with Flow Cytometry Staining Buffer and decant supernatant.
4. Analyze on flow cytometer or proceed with secondary staining on ice.

Note:

• Co-staining with CCL19-Fc may be affected by binding conditions and steric hindrance.
• 4B12 binding can block detectable CCL19-Fc binding.
• Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.
• Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CCR7 is a G protein-coupled receptor induced by Epstein-Barr virus (EBV) and expressed in blood, bone marrow, lymph node, and intestine. It regulates migration of memory T cells to inflamed tissues and promotes dendritic cell maturation. Ligands include CCL19 (ELC) and CCL21 (SLC). CCR family receptors (CCR1–CCR5) are critical for T cell chemotaxis and phagocyte migration to inflammation sites.

For Research Use Only. Not for use in diagnostic procedures.

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