Thermo Fisher Scientific CD127 Monoclonal Antibody (eBioRDR5), PerCP-Cyanine5.5, eBioscience
인간 CD127(IL-7Rα)을 인식하는 eBioRDR5 클론 단일클론 항체로, PerCP-Cyanine5.5 형광표지되어 있습니다. Flow cytometry에 최적화되어 있으며, IL-7 결합에 의해 차단됩니다. 인간 및 마우스 반응성, 고순도 친화크로마토그래피 정제, 연구용 전용 제품입니다.
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Thermo Fisher Scientific CD127 Monoclonal Antibody (eBioRDR5), PerCP-Cyanine5.5, eBioscience
Applications and Tested Dilution
- Immunohistochemistry (Frozen): Not specified
View 1 publication - Flow Cytometry: 5 µL (0.125 µg)/test
View 21 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-Cyanine5.5, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioRDR5 |
| Conjugate | PerCP-Cyanine5.5 |
| Excitation / Emission Max | 489 / 679 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10670349 |
Available Formats
Unconjugated, Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 561, Alexa Fluor 647, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV/Violet/Yellow series, eFluor 450/506/660, FITC, Functional Grade, NovaFluor series, PE, PE-Cyanine5/5.5/7, PE-eFluor 610, PerCP-eFluor 710, Super Bright series, and Custom Conjugation available.
Product Specific Information
The eBioRDR5 monoclonal antibody reacts with human CD127 (Interleukin-7 Receptor alpha). CD127 complexes with CD132 (common gamma chain) to form the IL-7 receptor. It is expressed by immature B cells, thymocytes, and most mature T cells, with transient down-regulation upon activation. IL-7 binding triggers Jak/STAT signaling pathways essential for lymphocyte development and T cell homeostasis.
CD127 expression is notably down-regulated in CD4+CD25+ regulatory T cells (Tregs), making it a useful marker to distinguish true Tregs enriched for Foxp3 expression and suppressive activity.
Binding of eBioRDR5 to PBMCs is blocked by recombinant human IL-7 (Product #14-1079-80).
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and validated for flow cytometry of normal human peripheral blood cells.
Use at 5 µL (0.125 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).
Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD127 (IL-7Rα) is a glycoprotein involved in lymphopoiesis regulation. The active IL7R complex is an alpha/gamma heterodimer; the gamma chain activates signal transduction, while the alpha chain mediates specific signaling. CD127 promotes proliferation of precursor B cells, thymocytes, and mature T cells. Dysfunctions are associated with severe combined immunodeficiency disorders.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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