Thermo Fisher Scientific CD127 Monoclonal Antibody (eBioRDR5), PerCP-Cyanine5.5, eBioscience

Thermo Fisher Scientific CD127 Monoclonal Antibody (eBioRDR5), PerCP-Cyanine5.5, eBioscience

Applications and Tested Dilution

• Immunohistochemistry (Frozen): Not specified
  View 1 publication
• Flow Cytometry: 5 µL (0.125 µg)/test
  View 21 publications

Product Specifications

Available Formats

Unconjugated, Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 561, Alexa Fluor 647, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV/Violet/Yellow series, eFluor 450/506/660, FITC, Functional Grade, NovaFluor series, PE, PE-Cyanine5/5.5/7, PE-eFluor 610, PerCP-eFluor 710, Super Bright series, and Custom Conjugation available.

Product Specific Information

The eBioRDR5 monoclonal antibody reacts with human CD127 (Interleukin-7 Receptor alpha). CD127 complexes with CD132 (common gamma chain) to form the IL-7 receptor. It is expressed by immature B cells, thymocytes, and most mature T cells, with transient down-regulation upon activation. IL-7 binding triggers Jak/STAT signaling pathways essential for lymphocyte development and T cell homeostasis.

CD127 expression is notably down-regulated in CD4+CD25+ regulatory T cells (Tregs), making it a useful marker to distinguish true Tregs enriched for Foxp3 expression and suppressive activity.

Binding of eBioRDR5 to PBMCs is blocked by recombinant human IL-7 (Product #14-1079-80).

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and validated for flow cytometry of normal human peripheral blood cells.
Use at 5 µL (0.125 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).
Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD127 (IL-7Rα) is a glycoprotein involved in lymphopoiesis regulation. The active IL7R complex is an alpha/gamma heterodimer; the gamma chain activates signal transduction, while the alpha chain mediates specific signaling. CD127 promotes proliferation of precursor B cells, thymocytes, and mature T cells. Dysfunctions are associated with severe combined immunodeficiency disorders.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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