Thermo Fisher Scientific NEFL Monoclonal Antibody (OTI3G2), TrueMAB
인간 NEFL 단백질(1-360aa)에 특이적인 Mouse IgG1 단클론 항체로 신경세포 내 중간 필라멘트 연구용. Western blot 및 IHC(P) 검증 완료. 무보존제, 동결건조 제형으로 안정적이며 재구성 및 결합 실험에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3G2 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–360 of human NEFL produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Neurofilaments are intermediate filament proteins found specifically in neurons, composed mainly of NF-L, NF-M, and NF-H.
NF-L (light chain) is the low molecular weight subunit (~70 kDa) involved in maintaining neuronal caliber.
NF-H (heavy chain) runs at 180–220 kDa on SDS-PAGE and varies across species.
Expression of these neuronal intermediate filament proteins is tissue-specific and developmentally regulated.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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