Thermo Fisher Scientific VIM Monoclonal Antibody (OTI1H10), TrueMAB

Applications

• Western Blot (WB)
  Tested Dilution: 1:200–1:1,000

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).

Target Information

Vimentin is a developmentally regulated intermediate filament protein found in cells of mesenchymal origin. It plays a role in intracellular protein transport between the nucleus and plasma membrane. Vimentin expression occurs early in cellular development and is later replaced by tissue-specific intermediate filament proteins. It is involved in steroid synthesis regulation through its function as a lipid droplet storage network. Phosphorylation of vimentin leads to filament breakdown and cytoskeletal rearrangements that promote cholesterol transport to mitochondria, enhancing steroid synthesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.