Thermo Fisher Scientific VIM Monoclonal Antibody (OTI1H10), TrueMAB
Human Vimentin 단백질(aa 210-466)에 특이적인 Mouse Monoclonal Antibody. Western blot 검증 완료. Lyophilized 형태로 제공되며 PBS buffer에 trehalose 포함. 연구용으로만 사용 가능.
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Applications
- Western Blot (WB)
Tested Dilution: 1:200–1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1H10 |
| Immunogen | Human recombinant protein fragment (aa 210–466 of human VIM) produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
Vimentin is a developmentally regulated intermediate filament protein found in cells of mesenchymal origin. It plays a role in intracellular protein transport between the nucleus and plasma membrane. Vimentin expression occurs early in cellular development and is later replaced by tissue-specific intermediate filament proteins. It is involved in steroid synthesis regulation through its function as a lipid droplet storage network. Phosphorylation of vimentin leads to filament breakdown and cytoskeletal rearrangements that promote cholesterol transport to mitochondria, enhancing steroid synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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