Thermo Fisher Scientific DNMT3A Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Target Information

Methylation of DNA at cytosine residues plays an important role in the regulation of gene expression, genomic imprinting, and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with cancer development.
Three families of mammalian DNA methyltransferase genes have been identified: Dnmt1, Dnmt2, and Dnmt3.

• Dnmt1: Constitutively expressed in proliferating cells; its inactivation causes global demethylation and embryonic lethality.
• Dnmt2: Expressed at low levels in adult tissues; inactivation does not affect DNA methylation.
• Dnmt3 family (Dnmt3a, Dnmt3b): Strongly expressed in ES cells; expression down-regulated during differentiation and low in adult somatic tissues.
  Mutations in the Dnmt3b gene are associated with the rare autosomal recessive disorder ICF syndrome (immunodeficiency, centromeric instability, and facial anomalies).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.