Thermo Fisher Scientific H4K20me3 Polyclonal Antibody
H4K20me3 Polyclonal Antibody는 인간, 마우스, 랫트에서 반응하며 Western blot, IHC, ICC/IF 등에 사용 가능. 항원 친화 크로마토그래피로 정제된 고품질 항체로, PBS 기반 보존액에 포함되어 안정적. 연구용으로 핵 단백질 히스톤 H4의 트라이메틸화된 Lys20 검출에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| Dot blot (DB) | 1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H4K20me3 (trimethyl Lys20) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792667 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (100%), Xenopus laevis (100%).
- Positive Control: HeLa, HeLa nuclear.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of chromosomal fiber in eukaryotes. Two molecules of each core histone (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units called nucleosomes. The linker histone H1 interacts with linker DNA between nucleosomes and functions in chromatin compaction.
This gene is intronless and encodes a member of the histone H4 family. Transcripts lack polyA tails but contain a palindromic termination element and are located in the large histone gene cluster on chromosome 6.
⚠ WARNING: This product can expose you to chemicals including mercury, known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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