Thermo Fisher Scientific GFP Monoclonal Antibody (GF28R), DyLight 800 4X PEG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 3 publications 3 publications

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

GF28R

Immunogen

GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugated.

Conjugate

DyLight™ 800 4X PEG DyLight™ 800 4X PEG DyLight™ 800 4X PEG

View additional formats

• Unconjugated
• Biotin
• DyLight 650
• DyLight 680
• HRP
• Request custom conjugation

Excitation/Emission Max

789/794 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

MES with proprietary stabilizer

Contains

no preservative

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2537650

Product Specific Information

MA5-15256-D800 has been successfully used for Western blotting. DyLight 800 4X PEG has an excitation/emission of 783/797 nm.

Target Information

The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.