Thermo Fisher Scientific GFP Monoclonal Antibody (GF28R), DyLight 680
GFP 단백질 검출용 DyLight 680 결합 단클론 항체. Western blot 및 면역형광(ICC/IF)에 적합. 높은 특이성과 재현성을 제공하며, 676/705 nm 형광 특성을 가짐. 단백질 A 정제, 4°C 보관, 연구용 전용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | View 3 publications |
| Immunocytochemistry (ICC/IF) | 1:25–1:100 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Published Species | Tag |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GF28R |
| Immunogen | GFP from the jellyfish Aequorea victoria N-terminal peptide-KLH conjugated |
| Conjugate | DyLight™ 680 |
| Excitation / Emission Max | 676 / 705 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.02% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2537649 |
Available Formats
Product Specific Information
MA5-15256-D680 has been successfully used in immunofluorescence and Western blot applications.
DyLight 680 has an excitation/emission of 692/712 nm.
Target Information
The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in its bioluminescence reaction. GFP is widely used as a reporter protein for gene expression and as a protein tag in cell culture and multicellular organisms.
As a fusion tag, GFP can be used to localize proteins, study protein movement, and investigate subcellular dynamics. GFP fluorescence is stable, species-independent, and suitable for various applications, including monitoring gene expression, protein localization, and protein–protein interactions (e.g., yeast two-hybrid, FRET).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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