Thermo Fisher Scientific GFP Monoclonal Antibody (GF28R)
Thermo Fisher Scientific의 GFP Monoclonal Antibody (GF28R)는 Aequorea victoria 유래 GFP를 인식하는 Mouse IgG1 항체로, EGFP, YFP, CFP 등 다양한 GFP 변형체에 반응합니다. WB, ELISA, ICC/IF, IP 등 다중 응용에 적합하며, 고순도 Protein A 정제 및 1 mg/mL 농도의 액상 형태로 제공됩니다.
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Thermo Fisher Scientific GFP Monoclonal Antibody (GF28R)
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 | 84 |
| Immunohistochemistry (IHC) | – | 12 |
| Immunohistochemistry (Paraffin) (IHC (P)) | – | 1 |
| Immunocytochemistry (ICC/IF) | 1:500–1:2,000 | 21 |
| Flow Cytometry (Flow) | – | 2 |
| ELISA | Assay-dependent | 1 |
| Immunoprecipitation (IP) | Assay-dependent | 16 |
| ChIP assay (ChIP) | – | 1 |
| Affinity Purification (AP) | – | 1 |
| Miscellaneous PubMed (Misc) | – | 2 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Tag |
| Published Species | Tag |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GF28R |
| Immunogen | GFP from the jellyfish Aequorea victoria N-terminal peptide-KLH conjugated |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2 |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10979281 |
Available Conjugated Formats
Product Specific Information
MA5-15256 recognizes native and denatured forms of GFP (Green Fluorescent Protein) and its variants including EGFP, YFP, EYFP, and CFP.
It is validated for immunoprecipitation, ELISA, Western blotting, and immunofluorescence applications.
Store at 4°C for short term; add 0.05% sodium azide if desired. For long term storage, store at -20°C and avoid freeze/thaw cycles.
Target Information
The jellyfish Aequorea victoria contains GFP that emits light in bioluminescence reactions.
GFP is widely used as a reporter protein and as a protein tag in both eukaryotic and prokaryotic organisms.
As a fusion tag, GFP enables localization studies, tracking protein movement, and investigation of subcellular compartment dynamics.
GFP is a 27 kDa monomeric protein that forms its fluorescent pigment autocatalytically, absorbing blue light (395 nm) and emitting green light (508 nm).
Its fluorescence is stable and species-independent, making it ideal for monitoring gene expression, protein localization, and studying protein interactions (e.g., FRET experiments).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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