Thermo Fisher Scientific GFP Monoclonal Antibody (GF28R), Biotin
GFP 단백질을 인식하는 Biotin 결합 단일클론 항체로, Western blot, ELISA, ICC/IF, IP 등에 적합합니다. Mouse IgG1 항체이며 Protein A로 정제되었습니다. 1 mg/mL 농도의 액상 형태로 4°C에서 보관합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | View 3 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | – | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:500–1:2,000 | – |
| ELISA | Assay-dependent | – |
| Immunoprecipitation (IP) | Assay-dependent | – |
Product Specifications
| Category | Specification |
|---|---|
| Species Reactivity | Tag |
| Published Species | Tag |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GF28R |
| Immunogen | GFP from the jellyfish Aequorea victoria N-terminal peptide-KLH conjugated |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.02% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2537647 |
Available Formats
Product Specific Information
MA5-15256-BTIN has been successfully used for Western blot, ELISA, ICC/IF, and IP applications.
Target Information
The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction. GFP is widely used as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and multicellular organisms. As a fusion tag, GFP helps localize proteins, study their movement, and analyze subcellular dynamics.
GFP is a 27 kDa monomeric protein that autocatalytically forms a fluorescent pigment. The wild-type protein absorbs blue light (395 nm) and emits green light (508 nm) without the need for additional cofactors. GFP fluorescence is stable, species-independent, and suitable for diverse applications including protein localization, interaction studies (yeast two-hybrid), and fluorescence energy transfer (FRET) experiments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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