Thermo Fisher Scientific RFP Monoclonal Antibody (RF5R)
Thermo Fisher Scientific의 RFP Monoclonal Antibody (RF5R)는 RFP 및 DsRed, mCherry, mOrange 등 다양한 변형체를 인식합니다. WB, IHC, ICC/IF, ELISA, IP 등에 적합하며, 단일클론 Mouse IgG1 형식으로 높은 특이성과 재현성을 제공합니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 | View 15 publications |
| Immunohistochemistry (IHC) | Assay-dependent | View 23 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 2 publications |
| Immunocytochemistry (ICC/IF) | 1:500–1:2,000 | View 9 publications |
| ELISA | Assay-dependent | View publications |
| Immunoprecipitation (IP) | Assay-dependent | View 4 publications |
| Miscellaneous PubMed (Misc) | - | View 2 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Tag |
| Published Species | Tag |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | RF5R |
| Immunogen | RFP from the Discosoma sea anemone N-terminal peptide-KLH conjugated |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2 |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10999796 |
Product Specific Information
MA5-15257 recognizes both native and denatured forms of Red Fluorescent Protein (RFP) and its variants, including DsRed, mCherry, and mOrange.
It has been validated in immunoprecipitation, ELISA, Western blot, immunohistochemistry, immunocytochemistry, and immunofluorescence applications.
For short-term storage, keep at 4°C. Add 0.05% sodium azide if desired. For long-term storage, store at -20°C and avoid repeated freeze/thaw cycles.
Target Information
Red Fluorescent Protein (RFP) is derived from Discosoma species (Uniprot ID: Q9U6Y8).
It is widely used as a molecular tag for studying protein localization, reporter gene expression, and protein-protein interactions.
Although naturally a homotetramer, monomeric variants have been engineered for diverse research applications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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