Thermo Fisher Scientific RFP Monoclonal Antibody (RF5R)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:3,000

View 15 publications 15 publications

Immunohistochemistry (IHC)

Assay-dependent

View 23 publications 23 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:500-1:2,000

View 9 publications 9 publications

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

View 4 publications 4 publications

Miscellaneous PubMed (Misc)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

RF5R

Immunogen

RFP from the Discosoma sea anemone N-terminal peptide-KLH conjugated.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10999796

Product Specific Information

MA5-15257 recognizes native and denatured forms of RFP (Red Fluorescent Protein) and its many variants: DsRed, mCherry, mOrange, etc.

MA5-15257 has successfully been used in immunoprecipitation, ELISA, Western blotting, immunohistochemistry, immunocytochemistry and immunofluorescence applications.

MA5-15257 may be stored at 4ºC short term. Add 0.05% sodium azide if desired. For long term storage, store at -20ºC, avoiding freeze/thaw cycles.`

Target Information

Red Fluorescent Protein (RFP) is isolated from sea anemone (Discosoma sp, Uniprot ID: Q9U6Y8), and commonly used as a molecular tag to study protein localization, reporter gene expression, and protein-protein interactions. Although it exists as a homotetramer, monomeric variants have been constructed for use in various applications.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.