Thermo Fisher Scientific Tau Monoclonal Antibody (3D4), FITC

Applications and Tested Dilutions

Product Specifications

Additional Formats

• APC (Cat. No. MA5-45954)
• PerCP (Cat. No. MA5-45956)
• Custom conjugation available upon request

Product Specific Information

A 1:1,000 dilution of MA5-45955 was sufficient for detection of Tau 2N4R P301S Fibril in 20 µg of Mouse Brain by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Detects multiple bands. Antibody detects monomer and fibril under native conditions (dot blot).

Target Information

Tau is a neuronal microtubule-associated protein found predominantly on axons. It promotes tubulin polymerization and stabilizes microtubules. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, functioning as a linker between both.
Axonal polarity is determined by TAU/MAPT localization in the neuronal cell body domain defined by the centrosome. Short isoforms allow cytoskeletal plasticity, while longer isoforms stabilize it.
In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building blocks of neurofibrillary lesions in Alzheimer’s disease (AD) brain. Hyper-phosphorylation impairs microtubule binding, leading to destabilization and neuronal degeneration.
Tau phosphorylation is mediated by multiple kinases including GSK-3β, PKA, cdk5, and casein kinase II. Hyper-phosphorylated Tau is associated with neurofibrillary lesions in Alzheimer’s disease and other neurodegenerative disorders such as Pick’s disease, frontotemporal dementia, corticobasal degeneration, and progressive supranuclear palsy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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