Thermo Fisher Scientific Tau Monoclonal Antibody (1D5), APC

Applications and Tested Dilutions

Product Specifications

Additional Formats

• FITC (Cat. No. MA5-45952)
• PerCP (Cat. No. MA5-45953)
• Custom conjugation available upon request

Product Specific Information

A 1:1,000 dilution of MA5-45951 was sufficient for detection of Tau 2N4R P301S Fibril in 20 µg of mouse brain by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Detects multiple bands. Antibody detects monomer under denaturing conditions but preferentially detects fibril under native conditions (dot blot).

Target Information

Tau is a neuronal microtubule-associated protein found predominantly on axons. It promotes tubulin polymerization and stabilizes microtubules. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, functioning as a linker between both.
Axonal polarity is determined by TAU/MAPT localization. Short isoforms allow cytoskeletal plasticity, while longer isoforms contribute to stabilization.
In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer’s disease. Hyper-phosphorylation impairs microtubule binding, leading to destabilization and neuronal degeneration.
Tau is phosphorylated by several kinases, including GSK-3β, PKA, cdk5, and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in Alzheimer’s disease, Pick’s disease, frontotemporal dementia, corticobasal degeneration, and progressive supranuclear palsy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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