Thermo Fisher Scientific Tau Monoclonal Antibody (1D5), FITC

Applications and Tested Dilution

Product Specifications

Additional Formats

• APC: MA5-45951
• PerCP: MA5-45953
• Custom conjugation available upon request

Product Specific Information

A 1:1,000 dilution of MA5-45952 was sufficient for detection of Tau 2N4R P301S Fibril in 20 µg of Mouse Brain by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Detects multiple bands. Antibody detects monomer under denaturing conditions but preferentially detects fibril under native conditions (dot blot).

Target Information

Tau is a neuronal microtubule-associated protein found predominantly on axons. It promotes tubulin polymerization and stabilizes microtubules. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, functioning as a linker between both. Tau localization contributes to axonal polarity.
Short isoforms provide cytoskeletal plasticity, while longer isoforms stabilize it. Hyper-phosphorylated Tau forms paired helical filaments (PHF), a hallmark of neurofibrillary lesions in Alzheimer’s disease. Hyper-phosphorylation impairs Tau’s microtubule-binding, leading to destabilization and neuronal degeneration.
Tau phosphorylation is mediated by kinases such as GSK-3β, PKA, CDK5, and casein kinase II. Hyper-phosphorylated Tau is also found in other neurodegenerative diseases including Pick’s disease, frontotemporal dementia, corticobasal degeneration, and progressive supranuclear palsy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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