Thermo Fisher Scientific Tau Monoclonal Antibody (1D5), FITC
Tau 단백질을 인식하는 FITC 표지 단일클론 항체로, WB, ICC/IF, ELISA, Dot blot 등 다양한 응용에 적합합니다. 인간, 마우스, 랫트 반응성이 있으며, 단량체와 섬유형 모두 검출 가능합니다. 단백질 G 정제, 4°C 어두운 곳 보관.
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Tau Monoclonal Antibody (1D5), FITC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| ELISA | Assay-dependent |
| Dot blot (DB) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1D5 |
| Immunogen | Human Recombinant Tau441 (2N4R), P301S mutant Protein Pre-formed Fibrils |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage conditions | 4°C, store in dark |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932406 |
Additional Formats
- APC: MA5-45951
- PerCP: MA5-45953
- Custom conjugation available upon request
Product Specific Information
A 1:1,000 dilution of MA5-45952 was sufficient for detection of Tau 2N4R P301S Fibril in 20 µg of Mouse Brain by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Detects multiple bands. Antibody detects monomer under denaturing conditions but preferentially detects fibril under native conditions (dot blot).
Target Information
Tau is a neuronal microtubule-associated protein found predominantly on axons. It promotes tubulin polymerization and stabilizes microtubules. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, functioning as a linker between both. Tau localization contributes to axonal polarity.
Short isoforms provide cytoskeletal plasticity, while longer isoforms stabilize it. Hyper-phosphorylated Tau forms paired helical filaments (PHF), a hallmark of neurofibrillary lesions in Alzheimer’s disease. Hyper-phosphorylation impairs Tau’s microtubule-binding, leading to destabilization and neuronal degeneration.
Tau phosphorylation is mediated by kinases such as GSK-3β, PKA, CDK5, and casein kinase II. Hyper-phosphorylated Tau is also found in other neurodegenerative diseases including Pick’s disease, frontotemporal dementia, corticobasal degeneration, and progressive supranuclear palsy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.