Controls, Cofactors, and Buffers - Cofactors
✨AI 추천 연관 상품
AI가 분석한 이 상품과 연관된 추천 상품들을 확인해보세요
연관 상품을 찾고 있습니다...
Controls, Cofactors, and Buffers - Cofactors
Enzyme Controls
Analysis of cytochrome P450 form specific metabolism requires use of the appropriate control preparation in order to eliminate the possibility of metabolism by enzymes native to the cell lines. Corning Life Sciences prepares control microsomes and cytosols using procedures identical to those used to prepare other microsomal products.
For P450 enzymes, control microsomes are available from both Sf9 and High Five (Hi5 or BTI-TN-5B1-4) insect cell preparations. All insect cell control microsomes are prepared from insect cells infected with wild-type baculovirus. Control preparations are also available for UGT, MAO, NAT, and CES Corning® Supersomes™ enzymes products.
Normalization of Protein Concentration
Differences in total protein concentration can affect the free concentration of substrate and hence enzyme kinetics. The available substrate concentration may be significantly lower than initially assumed for those substrates which have a large potential to bind nonspecifically to protein (J. Pharmacol. Exp. Ther. 283:46 [1997]; Drug Metab. Dispos. 25:1359 [1997]). Total protein concentration can be controlled and normalized in each incubation by the addition of control microsomes.
NADPH Regenerating System
NADPH is a necessary cofactor in many xenobiotic metabolism reactions. NADPH is required for the measurement of oxidase activity catalyzed by P450s, FMOs, NADPH-P450 OR, and many other oxidase enzymes. A common source of NADPH in an oxidase enzyme assay is an NADPH regenerating system which generates NADPH in situ using an enzymatic reaction. For example, glucose-6-phosphate dehydrogenase (G6PDH) will convert NADP+ to NADPH in the presence of the substrate glucose-6-phosphate (Glc-6-PO4).
The Corning Gentest™ NADPH regenerating system consists of two reagents, Solution A (NADP+ and Glc-6-PO4) and Solution B (G6PDH). Each reagent is sold separately. Combined, these two reagents form a NADPH regenerating system that can be used for all NADPH requiring oxidase assays (cDNA-expressed enzymes and liver fractions). At least 200 to 400 enzyme assays can be performed using one vial each of Solution A and B. The total number of assays that can be performed is dependent on a researcher`s experimental design.
Choose your specification
Cofactor Type
- NADPH Regenerating System, Solution A
- NADPH Regenerating System, Solution B
- UGT Reaction Mix System, Solution A
- UGT Reaction Mix System, Solution B
- Phosphate Buffer
Products
Product Number | Product Name | Qty./Pk | Qty./Cs | Brand | Price | Unit Size | |
---|---|---|---|---|---|---|---|
451200 | Corning® Gentest™ NADPH Regenerating System, Solution B, 1 mL | 1 / Pk | 1 / Ea | Corning® | per each | ||
451201 | Corning® Gentest™ Phosphate Buffer, 0.5 M, pH 7.4, 500 mL | 1 / Pk | 1 / Ea | Corning® | per each | ||
451220 | Corning® Gentest™ NADPH Regenerating System, Solution A, 5 mL | 1 / Pk | 1 / Ea | Corning® | per each | ||
451300 | Corning® Gentest™ UGT Reaction Mix, Solution A, 2 mL | 1 / Pk | 1 / Ea | Corning® | per each | ||
451320 | Corning® Gentest™ UGT Reaction Mix, Solution B, 5 mL | 1 / Pk | 1 / Ea | Corning® | per each |
🏷️Corning 상품 둘러보기
동일 브랜드의 다른 상품들을 확인해보세요

Corning
Corning 96-well Spheroid Microplates
254,000원

Corning
Corning 384-well High Content Screening Microplates with Glass Bottom
2,043,000원
Corning
Controls, Cofactors, and Buffers - Cofactors
65,000원

Corning
Bottle Top Vacuum Filters
104,000원

Corning
Advanced Polyethylene CentriStar™ Cap for 50 mL Centrifuge Tubes, Sterile
168,000원
배송/결제/교환/반품 안내
배송 정보
기본 배송비 |
| 교환/반품 배송비 |
|
---|---|---|---|
착불 배송비 |
| ||
교환/반품 배송비 |
|
결제 및 환불 안내
결제수단 |
|
---|---|
취소 |
|
반품 |
|
환급 |
|
교환 및 반품 접수
교환 및 반품 접수 기한 |
|
---|---|
교환 및 반품 접수가 가능한 경우 |
|
교환 및 반품 접수가 불가능한 경우 |
|
교환 및 반품 신청
교환 절차 |
|
---|---|
반품 절차 |
|