Thermo Fisher Scientific LIME1 Monoclonal Antibody (OTI9E9), TrueMAB
인간 LIME1 단백질(aa 30-295)을 면역원으로 제작된 Mouse IgG1 단일클론 항체. Western blot에 1:2,000 희석비로 검증됨. 동결건조 형태로 제공되며, PBS(pH 7.3, 8% 트레할로스) 완충액에 보존. -20°C에서 보관, 재동결/해동 피할 것.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI9E9 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 30–295 of human LIME1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with a highly basic cytoplasmic domain, expressed by T cells.
Upon CD4 or CD8 T cell coreceptor ligation, LIME is phosphorylated by Src-family kinases and associates with Lck, Fyn, and their regulator Csk.
Csk-mediated phosphorylation of LIME-associated Lck can enhance enzymatic activity, indicating LIME’s role as a positive regulator of TCR-dependent T cell signaling.
Under certain conditions, LIME may also mediate inhibitory signals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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