Thermo Fisher Scientific HLA-G Monoclonal Antibody (87G), PE, eBioscience
인간 HLA-G 단백질을 인식하는 단클론 항체로, Flow Cytometry 분석용으로 최적화됨. PE 형광 표지로 488–561 nm 레이저에 적합. 태반, 종양 및 면역조절 연구에 활용 가능. 4°C 보관, 동결 금지.
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Thermo Fisher Scientific HLA-G Monoclonal Antibody (87G), PE, eBioscience™
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (0.25 µg)/test
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Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 87G |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11151694 |
Additional Formats
Product Specific Information
The monoclonal antibody 87G recognizes human HLA-G, a nonclassical MHC molecule involved in immune inhibition.
HLA-G has seven isoforms, and 87G detects both HLA-G1 and soluble HLA-G5. Expression occurs mainly in fetal trophoblast cells, protecting the fetus from maternal immune attack.
HLA-G expression is also found in certain tumors (melanoma, breast carcinoma, renal carcinoma, CLL, AML, B-CLL), pancreatic islets, erythroid and endothelial progenitors, and thymic medulla.
HLA-G⁺ CD4⁺ or CD8⁺ cells act as regulatory cells with distinct cytokine profiles. Receptors include CD85j/ILT2, CD85d/ILT4, and CD158.
Recent studies suggest roles in transplant tolerance and immune regulation.
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested using stimulated U937 cells at 5 µL (0.25 µg)/test.
A test corresponds to staining a cell sample (10⁵–10⁸ cells) in 100 µL final volume.
Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
HLA-G is a class I heavy chain paralogue forming a heterodimer with β2-microglobulin.
It is expressed on fetal-derived placental cells and consists of an ~45 kDa heavy chain encoded by 8 exons:
- Exon 1: Leader peptide
- Exons 2–3: α1 and α2 domains (peptide-binding)
- Exon 4: α3 domain
- Exon 5: Transmembrane region
- Exon 6: Cytoplasmic tail
HLA and MHC antibodies are essential tools in Immunopeptidomics, enabling neoantigen identification and characterization via LC-MS/MS.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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