Thermo Fisher Scientific HLA-G Monoclonal Antibody (87G), PE, eBioscience

Thermo Fisher Scientific HLA-G Monoclonal Antibody (87G), PE, eBioscience™

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 5 µL (0.25 µg)/test
  View 8 publications

Product Specifications

Additional Formats

• Unconjugated
• APC
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The monoclonal antibody 87G recognizes human HLA-G, a nonclassical MHC molecule involved in immune inhibition.
HLA-G has seven isoforms, and 87G detects both HLA-G1 and soluble HLA-G5. Expression occurs mainly in fetal trophoblast cells, protecting the fetus from maternal immune attack.
HLA-G expression is also found in certain tumors (melanoma, breast carcinoma, renal carcinoma, CLL, AML, B-CLL), pancreatic islets, erythroid and endothelial progenitors, and thymic medulla.
HLA-G⁺ CD4⁺ or CD8⁺ cells act as regulatory cells with distinct cytokine profiles. Receptors include CD85j/ILT2, CD85d/ILT4, and CD158.
Recent studies suggest roles in transplant tolerance and immune regulation.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested using stimulated U937 cells at 5 µL (0.25 µg)/test.
A test corresponds to staining a cell sample (10⁵–10⁸ cells) in 100 µL final volume.
Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

HLA-G is a class I heavy chain paralogue forming a heterodimer with β2-microglobulin.
It is expressed on fetal-derived placental cells and consists of an ~45 kDa heavy chain encoded by 8 exons:

• Exon 1: Leader peptide
• Exons 2–3: α1 and α2 domains (peptide-binding)
• Exon 4: α3 domain
• Exon 5: Transmembrane region
• Exon 6: Cytoplasmic tail

HLA and MHC antibodies are essential tools in Immunopeptidomics, enabling neoantigen identification and characterization via LC-MS/MS.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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