Thermo Fisher Scientific Gata-3 Monoclonal Antibody (TWAJ), PE, eBioscience

Thermo Fisher Scientific Gata-3 Monoclonal Antibody (TWAJ), PE, eBioscience™

Applications and Tested Dilution

• Flow Cytometry (Flow): 5 µL (0.06 µg)/test
• Publications: View 58 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 700
• Brilliant UV 395, 563, 661
• Brilliant Violet 421, 480, 605, 711, 786
• eFluor 450, 660
• PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The monoclonal antibody TWAJ recognizes mouse and human Gata-3, a T cell-specific transcription factor crucial for thymic development and Th2 differentiation.
Expression during embryonic development occurs in the CNS, skin, mammary glands, and kidney. Homozygous knockout is embryonic lethal, indicating essential developmental roles.

In the thymus, Gata-3 is mainly expressed on CD4 single-positive cells. During Th2 differentiation, Gata-3 binds the IL-4 promoter and represses T-bet expression, inhibiting Th1 differentiation.

Alternative splice variants (50 and 45 kDa) are recognized by this antibody.
Staining requires the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00).
Cross-reactivity in rhesus monkeys has been reported.

Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Pre-titrated and tested on mouse thymocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523).
Refer to BestProtocols® for detailed staining procedure.
Recommended use: 5 µL (0.06 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Excitation: 488–561 nm
Emission: 578 nm
Laser: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

GATA3 is a zinc finger transcription factor that binds enhancer elements of all four T-cell receptor genes (α, β, γ, δ). It is abundantly expressed in T lymphocytes and regulates TCR gene activation.
The human GATA3 gene consists of six exons over 17 kb, encoding two zinc fingers conserved with GATA1. It plays a critical role in T-cell lineage specification and enhancer binding.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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