Thermo Fisher Scientific HLA-E Monoclonal Antibody (3D12HLA-E), PE, eBioscience

Thermo Fisher Scientific HLA-E Monoclonal Antibody (3D12HLA-E), PE, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.5 µg)/test
• Publications: View 15 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• APC
• Functional Grade
• Custom Conjugation Request

Product Specific Information

The monoclonal antibody 3D12 (HLA-E) recognizes human HLA-E, a member of the Human Leukocyte Antigen family.
HLA-E is an MHC Class IB molecule involved in NK cell recognition. Binding of peptides derived from other Class I leader sequences to HLA-E and β2-microglobulin complex leads to interaction with NKG2A on NK cells, protecting HLA-G-expressing cells from NK cell-mediated killing. HLA-E expression is ubiquitous.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
  • Recommended use: 5 µL (0.5 µg) per test
  • A test is defined as staining a cell sample in a final volume of 100 µL
  • Cell number: 10⁵–10⁸ cells/test (empirically determined)
• Excitation: 488–561 nm
• Emission: 578 nm
• Laser Compatibility: Blue, Green, Yellow-Green lasers
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

HLA-E belongs to the HLA class I heavy chain paralogues. This molecule is a heterodimer composed of a heavy chain and a light chain (β2-microglobulin).
The heavy chain (~45 kDa) gene contains 8 exons:

1. Exon 1: Leader peptide
2. Exons 2–3: α1 and α2 domains (peptide-binding)
3. Exon 4: α3 domain
4. Exon 5: Transmembrane region
5. Exons 6–7: Cytoplasmic tail

HLA-E binds a restricted subset of peptides derived from leader peptides of other class I molecules.
HLA and MHC antibodies are crucial in Immunopeptidomics, aiding identification and characterization of neoantigens via HPLC-MS/MS.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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