Thermo Fisher Scientific CD69 Monoclonal Antibody (FN50), Brilliant Violet 786, eBioscience

Applications

Tested Application

• Flow Cytometry (Flow)

Tested Dilution

• 5 µL (0.06 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 561
• Alexa Fluor 660
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395
• Brilliant UV 615
• Brilliant UV 661
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 650
• Brilliant Violet 711
• eFluor 450
• FITC
• NovaFluor Blue 610-70S
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 780
• Request custom conjugation

Product Specific Information

The FN50 monoclonal antibody reacts with human CD69, also known as very early activation antigen (VEA). CD69 is a ~30 kDa disulfide-linked dimer expressed on lymphocytes, monocytes, and platelets, and is rapidly upregulated upon activation.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested for flow cytometry on normal human peripheral blood cells. Recommended use at 5 µL (0.06 µg) per test (100 µL final volume).

Fluorochrome Information:
Brilliant Violet™ 786 (BV786) emits at 786 nm, suitable for cytometers with a 405 nm laser. Ensure instrument compatibility.

Staining Buffer Recommendation:
When using multiple polymer dye-conjugated antibodies (e.g., Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use:

• Super Bright Complete Staining Buffer (Product # SB-4401-42)
• Brilliant Stain Buffer™ (Product # 00-4409-75)

Light Sensitivity:
Protect vial and stained samples from light to avoid photo-induced oxidation.

Fixation:
Samples can be stored in:

• IC Fixation Buffer (Product # 00-8222-49)
• 1-step Fix/Lyse Solution (Product # 00-5333-54)
  for up to 3 days at 4°C in the dark.
• Not compatible with methanol-based fixation.*

Excitation: 407 nm
Emission: 786 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide-bonded homodimer (~60 kDa under non-reducing conditions). It contains N-linked oligosaccharides and is present on activated platelets. CD69 expression is induced upon T lymphocyte activation and may play a role in proliferation and signal transduction in NK cells and platelets.
Diseases associated with CD69 dysfunction include coccidiodomycosis and asthma.
Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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