Thermo Fisher Scientific CD117 (c-Kit) Monoclonal Antibody (2B8), Brilliant Violet 786, eBioscience

Thermo Fisher Scientific CD117 (c-Kit) Monoclonal Antibody (2B8), Brilliant Violet 786, eBioscience

Applications

• Tested Application: Flow Cytometry (Flow)
• Tested Dilution: 0.25 µg/test

Species Reactivity

• Mouse
• Pig

Product Specifications

Additional Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395, 563, 615, 661, 737
• Brilliant Violet 421, 480, 605, 650
• eFluor 450
• FITC
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation available

Product Specific Information

The 2B8 monoclonal antibody reacts with mouse CD117 (c-Kit receptor), also known as Steel factor receptor or stem cell factor receptor. This 145-kDa tyrosine kinase receptor is expressed in hematopoietic progenitor cells, mast cells, and plays a key role in signaling and activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Validated for flow cytometry on mouse bone marrow cells (≤0.25 µg/test)
• Recommended: Titrate for optimal performance

Brilliant Violet™ 786 (BV786) is a tandem dye emitting at 786 nm, suitable for cytometers with a violet (405 nm) laser.
Use Super Bright Complete Staining Buffer or Brilliant Stain Buffer™ to minimize non-specific interactions when using multiple polymer dye-conjugated antibodies.

Light Sensitivity: Protect vial and samples from light.
Fixation: Samples may be stored up to 3 days at 4°C in the dark using IC Fixation Buffer or 1-step Fix/Lyse Solution.
Note: BV786 is not compatible with methanol-based fixation.
Excitation: 407 nm; Emission: 786 nm; Laser: Violet Laser.

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company and used under license. Powered by Sirigen™.

Target Information

KIT (c-KIT) is a proto-oncogene encoding a type 3 transmembrane receptor for mast cell growth factor (stem cell factor, SCF). KIT regulates growth and activation of hematopoietic and non-hematopoietic cells.
Mutations in KIT are linked to gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. c-Kit activation triggers downstream signaling pathways (Ras/ERK, PI3K, PLC-γ, JAK/STAT) essential for hematopoiesis, stem cell maintenance, and gametogenesis.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

제품 이미지