Thermo Fisher Scientific CD80 (B7-1) Monoclonal Antibody (16-10A1), Brilliant Violet 786, eBioscience

Thermo Fisher Scientific CD80 (B7-1) Monoclonal Antibody (16-10A1), Brilliant Violet 786, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.25 µg/test
Publications: -

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 700
• APC
• Biotin
• Brilliant UV 615
• Brilliant UV 737
• Brilliant Violet 480
• Brilliant Violet 605
• Brilliant Violet 711
• eFluor 450
• FITC
• Functional Grade
• PE
• PE-Cyanine5
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation available

Product Specific Information

The 16-10A1 monoclonal antibody reacts with mouse CD80 (B7-1), a 55 kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells, activated B cells, and activated T cells. It binds with high affinity to CD28 and CD152 (CTLA-4), playing a crucial role in T-B cell communication and activation.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes at ≤0.25 µg/test (100 µL final volume). Optimal performance should be empirically determined.

Fluorochrome Information:
Brilliant Violet™ 786 (BV786) emits at 786 nm and is compatible with cytometers equipped with a 405 nm violet laser. Ensure instrument detection capability.

Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.

Light Sensitivity:
BV786 is sensitive to photo-induced oxidation. Protect from light.

Fixation:
Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54). BV786 is not compatible with methanol-based fixation.

Excitation: 407 nm
Emission: 786 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD80 (B7-1) and CD86 (B7-2) are ligands of CD28 and CTLA-4, critical for T cell activation. Both are expressed on antigen-presenting cells and can substitute for each other in costimulatory processes. CD80 is rapidly induced on activated B cells and various lymphoma cell lines. Dysfunction of CD80 is associated with diseases such as gallbladder squamous cell carcinoma and myocarditis.

For Research Use Only. Not for diagnostic procedures or resale without authorization.

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