Thermo Fisher Scientific CD282 (TLR2) Monoclonal Antibody (6C2), eFluor 660, eBioscience
마우스 TLR2(CD282)를 인식하는 6C2 클론의 라트 단클론 항체로 eFluor 660 형광체가 결합되어 있음. Flow Cytometry에 최적화되어 있으며, 0.125 µg/test로 사용 가능. 633 nm 레이저로 여기, 668 nm에서 방출. 4°C 암소 보관, 동결 금지.
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Thermo Fisher Scientific CD282 (TLR2) Monoclonal Antibody (6C2), eFluor 660, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 2 publications |
| Immunocytochemistry (ICC/IF) | - | View 3 publications |
| Flow Cytometry (Flow) | 0.125 µg/test | View 5 publications |
Product Specifications
| Specification | Description |
|---|---|
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), eFluor™ 660, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6C2 |
| Conjugate | eFluor™ 660 |
| Excitation/Emission Max | 651 / 668 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_11219871 |
Available Formats:
- Unconjugated
- Biotin
- FITC
- Functional Grade
- PE
- Custom conjugation available
Product Specific Information
The 6C2 monoclonal antibody reacts with mouse Toll-like receptor 2 (TLR2). Mouse TLR2 is expressed by the myeloid lineage, including macrophages and dendritic cells in splenocytes and the RAW264.7 cell line. TLR2 and TLR4 are pattern recognition receptors involved in innate immunity and inflammation.
Applications Reported:
This 6C2 antibody is suitable for flow cytometric analysis.
Applications Tested:
Validated in flow cytometric analysis of mouse splenocytes. Recommended usage ≤ 0.125 µg/test (100 µL final volume). Optimize antibody titration for best performance.
Fluorochrome Information:
eFluor® 660 replaces Alexa Fluor® 647.
- Excitation: 633–647 nm
- Emission: 668 nm
- Laser: Red Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
TLR2 is a member of the Toll-like receptor family, crucial for pathogen recognition and activation of innate immunity. It forms heterodimers with TLR1 or TLR6 to recognize bacterial and mycoplasma lipoproteins. TLR2 is abundantly expressed in peripheral blood leukocytes and mediates host responses to Gram-positive bacteria and yeast via NF-kappaB activation.
Expression is high in CD14+ monocytes, followed by CD15+ granulocytes, CD19+ B-cells, and CD3+ T-cells. Expression is modulated by immune factors such as LPS, GM-CSF, IL-1, IL-10 (upregulate), and IL-4, IFN-gamma, TNF (downregulate).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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