Thermo Fisher Scientific Granzyme B Monoclonal Antibody (NGZB), eFluor 660, eBioscience

Thermo Fisher Scientific Granzyme B Monoclonal Antibody (NGZB), eFluor 660, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• APC
• APC-eFluor 780
• eFluor 450
• FITC
• PE
• PE-Cyanine5.5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

This NGZB monoclonal antibody reacts with mouse Granzyme B, a member of the granzyme serine protease family found in cytotoxic T cells and NK cells. Granzyme B, also known as CGL1 or CTLA-1, is expressed only in activated cytotoxic T lymphocytes and plays a key role in apoptosis of target cells via mannose 6-phosphate receptor-mediated pathways.

• Applications Reported: Suitable for intracellular staining and flow cytometric analysis.
• Applications Tested: Validated using stimulated mouse splenocytes and the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00).
• Recommended usage ≤0.125 µg/test (100 µL final volume).
• eFluor® 660 is a replacement for Alexa Fluor® 647 and emits at 659 nm when excited with a 633 nm red laser.
• Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Granzyme B is a serine protease located in cytotoxic T cells and NK cell granules. It induces apoptosis in target cells through caspase-3 activation and mitochondrial pathways (via Bid cleavage). It interacts with mannose 6-phosphate receptor as a death receptor during cytotoxic T cell-mediated apoptosis. Multiple granzymes (A–G) have been identified in mouse CTLs and NK cells, while fewer have been cloned in humans.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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