Thermo Fisher Scientific Pan Cytokeratin Monoclonal Antibody (AE1/AE3), eFluor 660, eBioscience
다양한 세포 및 조직에서 사이토케라틴을 검출하는 AE1/AE3 클론 단일클론 항체. eFluor 660 형광 표지로 적색 레이저(633 nm)에서 검출 가능. IHC, ICC, WB 등 다양한 응용에 적합하며 인간 및 여러 동물 종에 반응. 연구용으로만 사용.
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Thermo Fisher Scientific Pan Cytokeratin Monoclonal Antibody (AE1/AE3), eFluor 660
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | View 1 publication |
| Immunohistochemistry (IHC) | - | View 7 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | View publications |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | View 1 publication |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | View 4 publications |
| Flow Cytometry (Flow) | - | View 3 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Non-human primate, Rabbit, Rhesus monkey |
| Published Species | Not Applicable |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | AE1/AE3 |
| Conjugate | eFluor™ 660 |
| Excitation/Emission Max | 651/668 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574300 |
Additional Formats:
- Alexa Fluor 488
- eFluor 570
- eFluor 615
- Custom conjugation available
Product Specific Information
The monoclonal antibodies AE1 and AE3 recognize many acidic and basic cytokeratin family members. Cytokeratins are intermediate filament proteins forming part of the cytoskeleton. They are expressed in epithelial cells and developmentally regulated. Many tumors express these proteins, aiding in identifying the origin of neoplasms.
- AE3 antibody recognizes 65–67 triplet, 64, 59, 58, 56, 54, and 52 kDa proteins (CK1–8).
- AE1 antibody recognizes 56.5, 54, 50, 48, and 40 kDa proteins (CK10, 14, 15, 16, 19).
Applicable to tissue samples from mouse, human, rat, primates (cynomolgus and rhesus), dog, cat, rabbit, and chicken.
Applications Reported:
Used for immunohistochemical staining, immunocytochemistry, and staining of frozen or paraffin-embedded tissue sections.
Applications Tested:
Tested by immunocytochemistry on methanol-fixed MCF7 cells. Recommended ≤5 µg/mL; titrate for optimal performance.
Fluorophore Information:
eFluor™ 660 replaces Alexa Fluor™ 647. Excitation: 633–647 nm; Emission: 668 nm; Laser: Red Laser.
Filtered post-manufacturing (0.2 µm).
Target Information
Cytokeratin pan is part of the intermediate filament protein family with high biochemical diversity. Represented by at least 20 different polypeptides in human epithelial tissues, molecular weight ranges from 40–68 kDa and isoelectric point from 4.9–7.8.
Cytokeratin subtype expression varies by epithelial type, differentiation stage, and disease state. These patterns assist in distinguishing epithelial malignancies and are valuable in tumor diagnosis, cytopathology, and flow cytometry assays.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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