Thermo Fisher Scientific IFN gamma Recombinant Mouse Monoclonal Antibody (D9D10)
인간 IFN-γ에 특이적으로 결합하는 재조합 마우스 단클론 항체로, WB, Flow, ELISA, IA 등 다양한 분석에 적용 가능. HEK293 세포 발현, Protein A 정제, 1 mg/mL 농도. 단기 4°C, 장기 -20°C 보관 권장.
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Thermo Fisher Scientific IFN gamma Recombinant Mouse Monoclonal Antibody (D9D10)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Flow Cytometry (Flow) | Assay-dependent |
| ELISA | Assay-dependent |
| Inhibition Assays (IA) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Baboon, Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | D9D10 |
| Immunogen | FPLC-purified natural human IFN-γ |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3074275 |
Product Specific Information
- Specificity: Binds human interferon gamma, a dimerized soluble cytokine that is the only member of the type II class of interferons.
Target Information
IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor and immune interferon produced primarily by T-lymphocytes and natural killer cells. It is a dimeric glycoprotein (~45 kDa) composed of two 146 amino acid subunits.
On SDS-PAGE, IFN gamma shows 25, 20, and minor 15.5 kDa bands due to differential glycosylation.
The biological activity of IFN gamma homodimer is highly species-specific; human IFN gamma does not cross-react with mouse.
Functions include:
- Antiviral and tumor antiproliferative activity
- Induction of MHC class I and II
- Macrophage activation
- Enhanced immunoglobulin secretion by B lymphocytes
IFN gamma acts through binding to IFN gamma receptor I and II, activating the JAK-STAT pathway. It shows about 40% sequence homology between human and mouse IFN gamma.
Upregulated by IL2, FGF basic, EGF; downregulated by vitamin D3 or DMN.
Mutations in the IFN gamma gene are associated with aplastic anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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